Abstract
Unlike classical β-1,4-galactosyltransferase (β-1,4-GalT I), β-1,4-GalT V (formerly IV*) has little activity towards 1 mM N-acetylglucosamine [Sato et al. (1998) Proc Natl Acad Sci USA 95: 472-477]. The human β-1,4-GalTs I and V were expressed individually in Sf-9 cells by transfection of the full coding sequences, and their N-acetyllactosamine synthetase activities were determined towards different N-acetylglucosamine concentrations. Kinetic studies using the cell homogenates as an enzyme source revealed that β-1,4-GalTs I and V possess Km values of 0.6 mM and 33 mM towards N-acetylglucosamine, and of 48 µM and 41 µM towards UDPGal, respectively. No significant inhibition of N-acetyllactosamine synthesis with α-lactalbumin was observed for β-1,4-GalT V but the significant inhibition with α-lactalbumin was observed for β-1,4-GalT I.
Similar content being viewed by others
References
Sheares BT, Carlson DM (1984) J Biol Chem 259: 8045-47.
Gleeson PA, Feeney J, Mills G, Hughes RC (1984) Eur J Biochem 144: 143-50.
Furukawa K, Matsuta K, Takeuchi F, Kosuge E, Miyamoto T, Kobata A (1990) Intern Immunol 2: 105-12.
Almeida R, Amado M, David L, Levery SB, Holmes E, Merkx G, Van Kessel AG, Rygaard E, Hassan H, Bennett E, Clausen H (1997) J Biol Chem 272: 31979-91.
Sato T, Furukawa K, Bakker H, Van den Eijnden DH, Van Die I (1998) Proc Natl Acad Sci USA 95: 472-77.
Lo N-W, Shaper JH, Pevsner J, Shaper NL (1998) Glycobiology 8: 517-26.
Schwientek T, Almeida R, Levery SB, Holmes EH, Bennett E, Clausen H (1998) J Biol Chem 273: 29331-40.
Sato T, Aoki N, Matsuda T, Furukawa K (1998) Biochem Biophys Res Comm 244: 637-41.
Nomura T, Takizawa M, Aoki J, Arai H, Inoue K, Wakisaka E, Yoshizuka N, Imokawa G, Dohmae N, Takio K, Hattori M, Matsuo N (1998) J Biol Chem 273: 13570-77.
Kodama S, Tsujimoto M, Tsuruoka N, Sugo T, Endo T, Kobata A (1993) Eur J Biochem 211: 903-08.
Glasgow LR, Paulson JC, Hill RL (1977) J Biol Chem 252: 8615-23.
Bhavanandan VP, Furukawa K (1995) In Biology of the Sialic Acids (Rosenberg A, ed) pp 145-96, New York: Plenum Publishing Co.
Morrison JF, Ebner KE (1971) J Biol Chem 246: 3992-98.
Hill RL, Brew K (1975) Adv Enzymol Rel Areas Mol Biol 43: 411-90.
Wieser RJ, Oesch F (1992) Trends Glycosci Glycotech 4: 160-67.
Perillo NL, Pace KE, Seilhamer JJ, Baum LG (1995) Nature 378: 736-39.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sato, T., Furukawa, K. Differences in N-acetyllactosamine synthesis between β-1,4-galactosyltransferases I and V. Glycoconj J 16, 73–76 (1999). https://doi.org/10.1023/A:1006957921382
Issue Date:
DOI: https://doi.org/10.1023/A:1006957921382