Abstract
In this study the effects of propionate, L-valine, L-isoleucine, and DL-methionine on the metabolism of D-3-hydroxybutyrate (D-3-HB) were investigated in the isolated perfused non-working rat heart.
Propionate inhibited the utilization (the total removal of D-3-HB by the heart) but stimulated the oxidation of D-3-HB. The degree of D-3-HB inhibition was dependent on the concentrations of propionate and D-3-HB. Furthermore, increasing the concentration of DL-hydroxybutyrate (DL-3-HB) to 16 or 30 mM abolished the inhibitory effect of propionate (4 mM). Whereas increasing the perfusion pressure from 40ndash;80 mmHg stimulated the utilization and the oxidation of D-3-HB; propionate (4 mM) severely inhibited the utilization of D-3-HB at 40 and 80 mmHg, when DL-3-HB was 5 mM. On the other hand insulin (2 mU .ml-1) stimulated the utilization and the oxidation of D-3-HB at perfusion pressure of 40 mmHg, but showed no effect at 80 mmHg. Insulin was unable to overcome the inhibitory effect of propionate. Propionate improved the oxidation but inhibited the utilization of D-3-HB, while L-valine and L-isoleucine showed no effects on the utilization and the oxidation of D-3-HB. DL-methionine inc d the utilization of D-3-HB by 14% without noticeable effects on the oxidation of D-3-HB. None of these anaplerotic substrates were suitable to ameliorate the utilization of D-3-HB.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Revsin-Lanier B, Lebowitz J, Morrow G III: Propionate enhancement of acetoacetate oxidation in perfused rat hearts. Clinical Science 59: 289–292, 1980
Taegtmeyer H: On the ability of ketone bodies to serve as the only energy providing substrate for rat heart at physiological work load. Basic Res Cardiol 78: 435–450, 1983
Taegtmeyer H, Hems R, Krebs HA: Utilization of energy providing substrates in the isolated working rat heart. Biochem J 186: 701–711, 1980
Russell RR, Taegtmeyer H: Changes in citric acid cycle flux and anaplerosis antedate the functional decline in isolated rat hearts utilizing acetoacetate. J Clin Invest 87: 384–390, 1991
Sultan AMN: D-3-hydroxybutyrate metabolism in the perfused rat heart. Molecular and Cellular Biochemistry 79: 113–118, 1988
Sultan AMN: The effect of fasting on D-3-hydroxybutyrate metabolism in the perfused rat heart. Mol Cell Biochem 93: 107–118, 1990
Sultan AMN: Effects of diabetes and insulin on ketone bodies metabolism in heart. Mol Cell Biochem 110: 17–23, 1992
Williamson JR, Krebs HA: Acetoacetate as fuel of respiration in the perfused rat heart. Biochem J 80: 540–547, 1961
Sultan AMN: The effects of chronic diabetes and physiological insulin concentration on ketone bodies metabolism in the heart. Diabetes Res Clin Exper 27: 47–60, 1994
Mellanby J, Williamson DH: Acetoacetate. In: Hans Ulrich Bergmeyer (ed). Methods of Enzymatic Analysis. Academic Press 4, 1974, pp 1841–1843
Williamson DH, Mellanby J: D-3-hydroxybutyrate. In: Hans Ulrich Bergmayer (ed). Methods of Enzymatic Analysis. Academic Press 4, 1974, pp 1836–1839
Fisher RB, O'Brien JA: The effects of endogenous and added insulin on the time-course of glucose uptake by the isolated perfused rat heart. Quart J Exp Physiol 57: 176–191, 1972
Krebs HA, Henseleit K: Untersuchungen uber die harnstoffbildung im tierkorper. Hoppe Seylers Z Physiol Chem 210: 33–66, 1932
Davis EJ, Quastel JH: The effects of short-chain fatty acids and starvation on the metabolism of glucose and lactate by the perfused guinea pig heart. Canad J Biochem 42: 1605–1621, 1964
Landaas S: Inhibition of branched-chain amino acid degradation by ketone bodies. Scand J Clin Lab Invest 37: 411–418, 1977
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sultan, A. The effects of anaplerotic substrates on D-3-hydroxybutyrate metabolism in the heart. Mol Cell Biochem 171, 59–64 (1997). https://doi.org/10.1023/A:1006865627489
Issue Date:
DOI: https://doi.org/10.1023/A:1006865627489