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Photosynthesis and associated metabolism during development of a Theobroma cacao hybrid with the lethal factor Luteus-Pa

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Photosynthetica

Abstract

The recessive lethal character Luteus-Pa, expressed as a yellowing of leaves of young seedlings and followed by death approximately 60 d after emergence, presents a 3:1 segregation in crosses and/or selfpollinated plants. We evaluated quantitatively the fluorescence emission of chlorophyll (Chl), gas exchange, and chemical composition of normal and recessive homozygous cacao seedlings of the cross Pa 121×Pa 169. The characteristics of Chl fluorescence kinetics were studied in stages B2, B3, C, D, and E of leaf development, corresponding to plant ages of 9 to 12, 13 to 15, 16 to 20, 21 to 30, and >30 d, respectively. Gas exchanges were measured in mature leaves of both seedlings. In regular intervals of 3 d beginning at 33 d after emergence, the seedlings were separated into roots, stems, leaves, and cotyledons to determine the contents of saccharides (SAC) and free amino acids (FAA) and variation of the leaf Chl content. The Chl distribution in complexes of the photosynthetic apparatus was analysed by SDS-PAGE in mature leaves of both normal and recessive 32-d-old seedlings. There were variations in Chl fluorescence, gas exchanges and chemical composition of different parts of both types of seedlings. However, no significant differences were found in the Chl distribution through photosynthetic complexes of 32-d-old normal and recessive homozygous seedlings. After that period a decrease in the Chl concentration was observed in the recessive seedlings, and only minimum fluorescence (F0) was found. The F0 values were higher in the recessive seedlings than in the normal ones. The net photosynthetic rate of mature leaves was negative in agreement with low conductance, transpiration rate, and high internal CO2 concentration. These factors might have contributed to a depletion in SAC in different plant parts. Although F0 partially reflects the Chl concentration in leaf tissue, the increase in its value was probably due to a damage in reaction centres of photosystem 2. Therefore, the growth and development of recessive homozygous seedlings depended exclusively on cotyledon reserves, the depletion of which leads to death.

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References

  • Anderson, J.M.: P-700 content and polypeptide profile of chlorophyll-protein complexes of spinach and barley thylakoids.-Biochim. biophys. Acta 591: 113–126, 1980.

    Google Scholar 

  • Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1–15, 1949.

    Google Scholar 

  • Baker, N.R., Bradbury, M.: Possible applications of chlorophyll fluorescence techniques for studying photosynthesis in vivo. In: Smith, H. (ed.): Plants and the Daylight Spectrum. Pp. 355–373. Academic Press, London-New York-San Francisco 1981.

    Google Scholar 

  • Baker, N.R., Hardwick, K.: Biochemical and physiological aspects of leaf development in cocoa (Theobroma cacao). I. Development of chlorophyll and photosynthetic activity.-New Phytol. 72: 1315–1324, 1973.

    Google Scholar 

  • Baker, N.R., Hardwick, K., Jones, P.: Biochemical and physiological aspects of leaf development in cocoa (Theobroma cacao). II. Development of chloroplast ultrastructure and carotenoids.-New Phytol. 75: 513–518, 1975.

    Google Scholar 

  • Baker, N.R., Webber, A.N., Bradbury, M., Markwell, J.P., Baker, M.G., Thornber, J.P.: Development of photochemical competence during growth of the wheat leaf.-In: Thornber, J.P., Stachelin, L.A., Hallick, R.B. (ed.): Biosynthesis of the Photosynthetic Apparatus: Molecular Biology, Development and Regulation. Pp. 237–255. A.R. Liss, New York 1984.

    Google Scholar 

  • Bartley, B.G.D.: Selfing of self-incompatible trees.-Annu. Rep. Cacao Res. (St. Augustine) 1968: 22–23, 1968.

    Google Scholar 

  • Bartley, B.G.D., Yamada, M.M., Castro, G.C.T., Melo, G.R.P.: [Genetics of Theobroma cacao: occurrence of lethal factor ‘Luteus-Pa’ in family Parinari.]-Theobroma 13(3): 275–278, 1983. [In Portugal.]

    Google Scholar 

  • Barton, R.: Fine structure of mesophyll cells in senescing leaves of Phaseolus.-Planta 71: 314–325, 1966.

    Google Scholar 

  • Beevers, L.: Senescence.-In: Varner, J.E., Bonner, J.D. (ed.): Plant Biochemistry. Pp. 771–794. Academic Press, New York 1976.

    Google Scholar 

  • Bolhár-Nordenkampf, H.R., Oquist, G.: Chlorophyll fluorescence as a tool in photosynthesis research.-In: Hall, D.O., Scurlock, J.M.O., Bolhàr-Nordenkampf, H.R., Leegood, R.C., Long, S.P. (ed.): Photosynthesis and Production in a Changing Environment: A Field and Laboratory Manual. Pp. 193–206. Chapman & Hall, London-Glasgow-New York-Tokyo-Melbourne-Madras 1993.

    Google Scholar 

  • Bruinsma, J.: A comment on the spectrophotometric determination of chlorophyll.-Biochim. biophys. Acta 52: 576–578, 1961.

    Google Scholar 

  • Daley, P.F., Raschke, K., Hall, J.T., Berry, J.A.: Topography of photosynthetic activity of leaves obtained from video images of chlorophyll fluorescence.-Plant Physiol. 90: 1233–1238, 1989.

    Google Scholar 

  • Danielson, C.E.: The breakdown of high molecular reserve proteins of peas during germination.-Acta chem. scand. 5: 551–554, 1951.

    Google Scholar 

  • Demmig, B., Björkman, O.: Comparison of the effect of excessive light on chlorophyll fluorescence (77 K) and photon yield of O2 evolution in leaves of higher plants.-Planta 171: 171–184, 1987.

    Google Scholar 

  • Demmig, B., Winter, K.: Light response of CO2 assimilation, reduction state of Q, and radiationless energy dissipation in intact leaves.-Aust. J. Plant Physiol. 15: 151–162, 1988.

    Google Scholar 

  • Ehleringer, J., Björkman, O.: Quantum yields for CO2 uptake in C3 and C4 plants. Dependence on temperature, CO2 and O2 concentration.-Plant Physiol. 59: 86–90, 1977.

    Google Scholar 

  • Filner, P.: Regulation of nitrate reductase in cultured tobacco cells.-Biochim. biophys. Acta 118: 229–310, 1966.

    Google Scholar 

  • Fondy, B.R., Geiger, D.R.: Effect of rapid changes in sink-source ratio on export and distribution of products of photosynthesis in leaves of Beta vulgaris L. and Phaseolus vulgaris L.-Plant Physiol. 66: 945–949, 1980.

    Google Scholar 

  • Fondy, B.R., Geiger, D.R.: Diurnal pattern of translocation and carbohydrate metabolism in source leaves of Beta vulgaris L.-Plant Physiol. 70: 671–676, 1982.

    Google Scholar 

  • Fong, F., Heath, R.L.: Age dependent changes in phospholipids and galactolipids in primary bean leaves (Phascolus vulgaris).-Phytochemistry 16: 215–217, 1977.

    Google Scholar 

  • Franceschi, V.R., Giaquinta, R.T.: The paraveinal mesophyll of soybean leaves in relation to assimilate transfer and compartmentation. II. Structural, metabolic and compartmental changes during reproductive growth.-Planta 157: 422–431, 1983.

    Google Scholar 

  • Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87–92, 1989.

    Google Scholar 

  • Horton, P., Black, M.T.: A comparison between cation and protein phosphorylation effects on the fluorescence induction curve in chloroplasts treated with 3-(3,4-dichlorophenyl)-1,1-dimethylurea.-Biochim. biophys. Acta 722: 214–218, 1983.

    Google Scholar 

  • Huber, S.C.: Relation between photosynthetic starch formation and dry weight partitioning between the shoot and the root.-Can. J. Bot. 61: 2709–2716, 1983.

    Google Scholar 

  • Jenkins, G.I., Baker, N.R., Bradbury, M., Woolhouse, H.W.: Photosynthetic electron transport during senescence of the primary leaves of Phaseolus vulgaris L. III. Kinetics of chlorophyll fluorescence emission from intact leaves.-J. exp. Bot. 32: 999–1008, 1981.

    Google Scholar 

  • Krall, J.P., Edwards, G.E.: Relationship between photosystem II activity and CO2 fixation in leaves.-Physiol. Plant. 86: 180–187, 1992.

    Google Scholar 

  • Kranse, G.H.: Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms.-Physiol. Plant. 74: 566–574, 1988.

    Google Scholar 

  • Krause, G.H., Behrend, U.: ΔpH-dependent chlorophyll fluorescence quenching indicating a mechanism of protection against photoinhibition of chloroplasts.-FEBS Lett. 200: 298–302, 1986.

    Google Scholar 

  • Krause, G.H., Laasch, H., Weis, E.: Regulation of thermal dissipation of absorbed light energy in chloroplast indicated by energy-dependent fluorescence quenching.-Plant Physiol. Biochem. 26: 445–452, 1988.

    Google Scholar 

  • Krause, G.H., Somersalo, S., Zumbusch, E., Weyers, B., Laasch, H.: On the mechanism of photoinhibition in chloroplasts. Relationship between changes in fluorescence and activity of photosystem II.-J. Plant Physiol. 136: 472–479, 1990.

    Google Scholar 

  • Krause, G.H., Vernotte, C., Briantais, J.-M.: Photoinduced quenching of chlorophyll fluorescence in intact chloroplasts and algac. Resolution into two components.-Biochim. biophys. Acta 679: 116–124, 1982.

    Google Scholar 

  • Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics.-Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313–349, 1991.

    Google Scholar 

  • McCready, R.M., Guggolz, J., Silveira, V., Owens, H.S.: Determination of starch and amylose in vegetables.-Anal. Chem. 22: 1156–1158, 1950.

    Google Scholar 

  • McKee, H.S.: Nitrogen metabolism of seedlings.-In: Ruhland, W. et al. (ed.): Encyclopedia of Plant Physiology. Vol. VIII. Pp. 477–515. Springer-Verlag, Berlin-Heidelberg-Göttingen 1958.

    Google Scholar 

  • McKersie, B.D., Thompson, J.E.: Phase behavior of chloroplast and microsomal membranes during leaf senescence.-Plant Physiol. 61: 639–643, 1978.

    Google Scholar 

  • Merkel, U., Muller, M.W., Minar, P.S., Biehl, B.: Light intensity influence on the characteristics of the photosynthetic apparatus from cocoa tree (Theobroma cacao L.) during leaf development.-Proc. 11th int. Cocoa Res. Conf. (Lagos) 1993: 645–653, 1994.

    Google Scholar 

  • Miranda, V., Baker, N.R., Long, S.P.: Limitations of photosynthesis in different regions of the Zea mays leaf.-New Phytol. 89: 179–190, 1981.

    Google Scholar 

  • Moore, S., Stein, W.N.: Photometric method for use in the chromatography of amino acids.-J. biol. Chem. 176: 367–388, 1948.

    Google Scholar 

  • Ögren, E., Baker, N.R.: Evaluation of a technique for the measurement of chlorophyll fluorescence from leaves exposed to continuous white light.-Plant Cell Environ. 8: 539–547, 1985.

    Google Scholar 

  • Scholes, J.D., Farrar, J.F.: Photosynthesis and chloroplast functioning within individual pustules of Uromyces muscari on bluebell leaves.-Physiol. Plant Pathol. 27: 387–400, 1985.

    Google Scholar 

  • Silvius, J.E., Chatterton, N.J., Kremer, D.F.: Photosynthate partitioning in soybean leaves at two irradiance levels. Comparative responses of acclimated and unacclimated leaves.-Plant Physiol. 64: 872–875, 1979.

    Google Scholar 

  • Stuhlfauth, T., Sültemeyer, D.F., Wein, S., Fock, H.P.: Fluorescence quenching and gas exchange in a water stressed C3 plant, Digitalis lanata.-Plant Physiol. 86: 246–250, 1988.

    Google Scholar 

  • Thorne, J.H., Koller, H.R.: Influence of assimilate demand on photosynthesis, diffusive resistances, translocation, and carbohydrate levels of soybean leaves.-Plant Physiol. 54: 201–207, 1974.

    Google Scholar 

  • Weis, E., Berry, J.A.: Quantum efficiency of photosystem II in relation to energy dependent quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 894: 198–208, 1987.

    Google Scholar 

  • Yamada, M.M., Bartley, B.G.D., Castro, G.C.T., Melo, G.R.P.: [Heredity of comparable factor in Theobroma cacao L. I. Phenotypic relations in family Pa (Parinari).]-Theobroma 12(3): 163–167, 1982. [in Portugal.].

    Google Scholar 

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Authors and Affiliations

  1. CEPLAC/CEPEC/SEFIS, Caixa Postal 7, Itabuna, BA, 45600-000, Brazil

    A.-A.F. De Almeida

  2. Botanisches Institut und Botanischer Garten der TU Braunschweig, Mendelssohnstr. 4, D-38092, Braunschweig, Germany

    R.R. Valle & P. Serrano Minar

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  1. A.-A.F. De Almeida
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  2. R.R. Valle
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  3. P. Serrano Minar
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De Almeida, AA., Valle, R. & Minar, P.S. Photosynthesis and associated metabolism during development of a Theobroma cacao hybrid with the lethal factor Luteus-Pa. Photosynthetica 35, 47–60 (1998). https://doi.org/10.1023/A:1006861513830

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