Abstract
The localization of the membrane-bound cyclic 3′,5′-AMP phosphodiesterasein cardiac tissues of both, rat and dog was studied by cytochemical method.40 µm thick slices from glutaraldehyde fixed heart tissue wereincubated in the medium with cAMP as a substrate and Pb ions as a capturemetal of the reaction product. The cAMP-PDE activity in the rat ventriclewas only shown positive on the sarcolemma. Whereas, in canine ventriculartissue the cAMP-PDE activity in cardiomyocytes was shown on the sarcolemma,on the junctional sarcoplasmic reticulum and on subsarcolemmal cisternae.The results confirm differences in the localization of cAMP-PDE in dog andrat heart.
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Okruhlicová, L., Tribulová, N., Styk, J. et al. Species differences in localization of cardiac cAMP-phosphodiesterase activity: A cytochemical study. Mol Cell Biochem 173, 183–188 (1997). https://doi.org/10.1023/A:1006845513962
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DOI: https://doi.org/10.1023/A:1006845513962