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Changes of the photosystem 2 activity and thylakoid proteins in spruce seedlings during water stress

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Photosynthetica

Abstract

We subjected spruce [Picea glauca (Moench) Voss × P. engelmanni Parry hybrid complex] seedlings to a severe water stress (shoot water potential ≤-3.5 MPa) to permit assessment of stress effects on photosystem 2 (PS2) in isolated photosynthetic membranes. The thylakoids and Triton-treated membranes isolated from stressed seedlings showed declines in O2-evolving capacity (H2O → 2,6-dichloro-p-benzoquinone, DCBQ) and electron transport activity (H2O → 2,6-dichlorophenol indophenol, DCIP). A partial restoration of O2-evolution by adding CaCl2 suggested an effect of water stress on the oxygen-enhancing extrinsic (OEE) polypeptides. Water stress had an additional impact on the reaction centre, shown by the inability of 1,5-diphenylcarbazide (DPC) to restore the electron transport (DPC → DCIP) to the levels seen in control membranes. Quantification of specific photosynthetic membrane proteins by immunoblots strengthened the above suggestions: after drought stress, concentrations of OEE1 and OEE2 declined by 40 %, and amount of the reaction centre protein D1, ATP synthetase, and cytochrome f also declined. The specific effect of stress on these proteins was confirmed by the fact that the amount of chlorophyll-protein complex CP2 was unchanged in membranes isolated from drought-stressed seedlings.

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Authors and Affiliations

  1. Forest Biotechnology Centre, British Columbia Research Inc., Vancouver B.C, V6S 2L2, Canada

    P.A.K. Eastman

  2. Department of Botany, University of British Columbia, Vancouver, B.C, V6T 1Z4, Canada

    P.A.K. Eastman & E.L. Camm

  3. Pest Management Branch, Alberta Environmental Centre, Vegreville, Alberta, T9C 1T4, Canada

    A. Rashid

  4. Department of Biology, University College of Fraser Valley, Abbotsford, B.C, V2S 7M9, Canada

    E.L. Camm

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  1. P.A.K. Eastman
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  2. A. Rashid
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  3. E.L. Camm
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Eastman, P., Rashid, A. & Camm, E. Changes of the photosystem 2 activity and thylakoid proteins in spruce seedlings during water stress. Photosynthetica 34, 201–210 (1998). https://doi.org/10.1023/A:1006836422855

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