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Quantitation of fumonisins in corn by HPLC with o-phthalaldehyde postcolumn derivatization and their identification by LC/MS

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Abstract

Fumonisins B1(FB1) and B2(FB2) were isocratically separated on a fluorocarbon column without using an ion pair reagent and nonvolatile buffer during the HPLC and were detected by an o-phthalaldehyde postcolumn derivatization system using a fluorescence detector. The minimum detectable concentrations of FB1 and FB2 in corn by this system were 0.01 μg/g and 0.01 μg/g, respectively. The separated fumonisins were further identified by a directly interfaced ion trap MS using electrospray ionization. FB1 and FB2 in naturally contaminated corn were identified in the selective ion monitoring mode at concentrations of 3.75μg/g and 1.44 μg/g, respectively.

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References

  1. Gelderblom WCA, Jaskiewicz K, Marasas WFO, Thiel PG, Horak RM, Vleggaar R, Kriek NPJ. Fumonisins novel mycotoxins with cancer promoting activity produced by Fusarium moniliforme. Appl Environ Microbiol 1988; 54: 1806–1811.

    PubMed  CAS  Google Scholar 

  2. Wilson TM, Ross PF, Owens DL, Rice LG, Green SA, Jenkins SJ, Nelson HA. Experimental reproduction of ELEM. Mycopathologia 1992; 117: 115–120.

    Article  PubMed  CAS  Google Scholar 

  3. Kellerman TS, Marasas WFO, Thiel PG, Gelderblom WCA, Cawood M, Coetzer JAW. Leukoencephalomalacia in two horses induced by oral dosing of fumonisin B1. Onderstepoort J Vet Res 1990; 57: 269–275.

    PubMed  CAS  Google Scholar 

  4. Harrison LR, Colvin BM, Greene JT, Newman LE, Cole JR. Pulmonary edema and hydrothorax in swine produced by fumonisin B1, a toxic metabolite of Fusarium moniliforme. JVet Diagn Invert 1990; 2: 217–221.

    CAS  Google Scholar 

  5. Sydenham EW, Thiel PG, Marasas WFO, Shephard GS, Van Schalkwyk DJK, Koch KR. Natural occurrence of some Fusarium mycotoxins in corn from low and high esophageal cancer prevalence areas of the Transkei, southern Africa. J Agric Food Chem 1990; 38: 1900–1903.

    Article  CAS  Google Scholar 

  6. Scott PM. Fumonisins. Intern J Food Microbiol 1993; 18: 257–270.

    Article  CAS  Google Scholar 

  7. Ross PF, Rice LG, Plattner RD, Osweiler GD, Wilson TM, Owens DL, Nelson HA, Richard JL. Concentrations of fumonisin B1 in feeds-associated animal health problems. Mycopathologia 1991; 114: 129–135.

    Article  PubMed  CAS  Google Scholar 

  8. Sydenham EW, Shephard GS, Thiel PG, Marasas WFO, Stockenstrom S. fumonisin contamination of commercial corn-based human foodstuffs. J Agric Food Chem 1991; 39: 2014–2018.

    Article  CAS  Google Scholar 

  9. Murphy PA, Rice LD, Ross PF. Fumonisin B1, B2 and B3 content of Iowa, Wisconsin, and Illinois corn and corn screenings. J Agric Food Chem 1993; 41: 263–266.

    Article  CAS  Google Scholar 

  10. Hopmans EC, Murphy PA. Detection of fumonisin B1, B2 and B3 and hydrolyzed fumonisin B1 in corn-containing foods. J Agric Food Chem 1993; 41: 1655–1658.

    Article  CAS  Google Scholar 

  11. Sydenham EW, Gelderblom WCA, Thiel PG, Marasas WFO. Evidence for the natural occurrence of fumonisin B1, a mycotoxin produced by Fusarium moniliforme, in corn. J Agric Food Chem 1990; 38: 285–290.

    Article  CAS  Google Scholar 

  12. Shephard GS, Sydenham EW, Thiel PG, Gelderblom WCA. Quantitative determination of fumonisins B1 and B2 by high-Performance liquid chromatography with fluorescence detection. J Liq Chromatogr 1990; 13: 2077–2087.

    CAS  Google Scholar 

  13. Scott PM, Lawrence GA. Liquid chromatographic determination of fumonisins with 4-fluoro-7-nitrobenzofurazan. J Assoc Off Anal Chem 1992; 75: 829–834.

    CAS  Google Scholar 

  14. Holcomb M, Thompson HC, Hankins J. Analysis of fumonisin B1 in rodent feed by gradient elution HPLC using precolumn derivatization with FMOC and fluorescence detection. J Agric Food Chem 1993; 41: 764–767.

    Article  CAS  Google Scholar 

  15. Bennett GA, Richard JL. Liquid chromatographic method for analysis of the naphthalene dicarboxaldehyde derivative of fumonisins. J AOAC Int 1994; 77: 501–506.

    CAS  Google Scholar 

  16. Akiyama H, Miyahara M, Toyoda M, Saito Y. Liquid chromatographic determination of fumonisins B1 and B2 in corn by precolumn derivatization with 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F). J Food Hyg Soc Japan 1995; 36: 77–81.

    CAS  Google Scholar 

  17. Miyahara M, Akiyama H, Toyoda M, Saito Y. New procedure for fumonisin B1 and B2 in corn and corn products by ion pair chromatography with o-phthaldialdehyde postcolumn derivatization and fluorometric detection. J Agric Food Chem 1996; 44: 842–847.

    Article  CAS  Google Scholar 

  18. Shephard GS, Thiel PG, Sydenham EW, Vleggaar R, Alberts JF. Determination of the mycotoxin fumonisin B1 and identi fication of its partially hydrolyzed metabolites in the faces of non-human primates. Fd Chem Toxic 1994; 32: 23–29.

    Article  CAS  Google Scholar 

  19. Plattner RD. Detection of fumonisins produced in Fusarium moniliforme cultures by HPLC with electrospray MS and evaporative light scattering detectors. Natural Toxins 1995; 3: 294–298.

    PubMed  CAS  Google Scholar 

  20. Josephs JL. Detection and characterization of fumonisin mycotoxins by liquid chromatography/electrospray ionization using ion trap and triple quadrupole mass spectrometry. Rapid Commun Mass Spectrom. 1996; 10: 1333–1344.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. National Institute of Health Sciences, 1-18-1, Kamiyoga, Setagaya, Tokyo, 158, Japan

    Hiroshi Akiyama, Makoto Miyahara, Yukihiro Goda & Masatake Toyoda

  2. Department of Medical Sciences, 693, Bumrungmueng Rd., Yod-se Bangkok, 10100, Thailand

    Mayuree Uraroongroj

Authors
  1. Hiroshi Akiyama
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  2. Mayuree Uraroongroj
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  3. Makoto Miyahara
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  4. Yukihiro Goda
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  5. Masatake Toyoda
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Akiyama, H., Uraroongroj, M., Miyahara, M. et al. Quantitation of fumonisins in corn by HPLC with o-phthalaldehyde postcolumn derivatization and their identification by LC/MS. Mycopathologia 140, 157–161 (1997). https://doi.org/10.1023/A:1006818226376

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