Skip to main content
SpringerLink
Log in

Cell suspension culture and plant regeneration in the latex-producing plant, Calotropis gigantea (Linn.) R. Br.

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

A procedure is outlined for the establishment of a proliferating cell suspension culture and subsequent plant regeneration of the latex-producing plant,Calotropis gigantea (Linn.) R. Br. Friable calluses were obtained by culturing hypocotyl explants on modified Murashige and Skoog medium with 2.69 μM α-naphthaleneacetic acid and 4.44 μM 6-benzyl-aminopurine. Friable calluses were transferred to modified Murashige and Skoog liquid medium containing 500 mg l−1 casein hydrolysate, 5% (v/v) coconut water and 5% (w/v) sucrose to initiate suspension cultures. Suspensions were subcultured every 10–12 days and supplemented with 13.56 μM 2,4-dichlorophenoxyacetic acid (2,4-D). After 3–4 subcultures, suspensions consisted of small, highly cytoplasmic cell clumps and large vacuolate cells. Plating the suspension clumps on medium containing 4.52 μM 2,4-dichlorophenoxyacetic acid and culturing in darkness produced macroscopic calluses, which subsequently produced a high number of shoots when placed in light and supplemented with 2.22 μM 6-benzyl-aminopurine and 0.45 μM 2,4-dichlorophenoxyacetic acid. Shoots were rooted using Bonner's solution containing 0.49 μM indole-3-butyric acid, and the plants successfully transferred to soil.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ammirato PV (1984) Induction, maintenance and manipulation of development in embryogenic cell suspension cultures. In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol 1 (pp 139–151). Academic Press, London

    Google Scholar 

  • Bonner J (1942) Culture of isolated roots of Acacia melanoxylon. Bull. Torrey Bot. Club 69: 130–133

    Article  Google Scholar 

  • Bunchanan RA, Cull IM, Otey FH & Russell CR (1978) Hydrocarbon and rubber producing crops. Evaluation of US plant species. Econ. Bot. 32: 131–146

    Google Scholar 

  • Burger DW, Liu L, Zary KW & Lee CI (1990) Organogenesis and plant regeneration from immature embryos of Rosa hybrida L. Plant Cell Tiss. Org. Cult. 21: 147–152

    Article  Google Scholar 

  • Calvin M (1979) Petroleum plantations for fuel and materials. Biosci. 29: 533–538

    Article  Google Scholar 

  • Chen Z, Qian C, Qin M, Xu X & Xiao Y (1982) Recent advances in anther culture of Hevea brasiliensis (Muell-Arg). Theor. Appl. Genet. 62: 103–108

    Article  Google Scholar 

  • Dutta SK & De S (1983) In vitro study of ovary induced callusing, rhizogenesis and presence of cardemolides in Calotropis gigantea (Linn.) R. Br. Tissue Cell Chrom. Res. 6: 10–12

    Google Scholar 

  • Dunbar KB, Kathryn JW, Petersen BH & Biesboer DD (1986) Identification of laticiferous in embryoids derived from callus and suspension cultures of Asclepias species (Asclepiadaceae). Am. J. Bot. 73: 847–851

    Article  Google Scholar 

  • Flick CE, Evans DA & Sharp WR (1983) Organogenesis. In: Evans DA, Sharp WR, Ammirato PV & Yamada Y (eds) Handbook of Plant Cell Culture (pp 13–81). Macmillan Publishing Co., New York

    Google Scholar 

  • Georges D, Chenieux JC & Ochatt SJ (1993) Plant regeneration from aged-callus of the woody ornamental species, Lonicera japonica cv ‘Hall's Prolific’. Plant Cell Rep. 13: 91–94

    CAS  Google Scholar 

  • Ho W & Vasil IK (1983) Somatic embryogenesis in sugarcane (Saccharum officinarum L.): Growth and plant regeneration from embryogenic cell suspension cultures. Ann. Bot. 51: 719–726

    Google Scholar 

  • Larkin PJ (1976) Purification and viability determination of plant protoplasts. Planta 128: 213–216

    Article  Google Scholar 

  • Meyer HJ & Vanstaden J (1988) The in vitro culture of Gerbera aurantiaca. Plant Cell Tiss. Org. Cult. 14: 25–30

    Article  Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Article  CAS  Google Scholar 

  • Nessler CL (1982) Somatic embryogenesis in the opium poppy Papaver somniferum L. Physiol. Plant. 55: 453–458

    Article  Google Scholar 

  • Ozawa K, Ling DH & Komamine A (1996) High frequency somatic embryogenesis from small suspension-cultured clusters of cells interspecific hybrid of Oryza. Plant Cell Tiss. Org. Cult. 46: 157–159

    Article  Google Scholar 

  • Park YG & Son SH (1988) Regeneration of plantlets from cell suspension derived callus of white poplar (Populus alba L.). Plant Cell Rep. 7: 567–570

    Article  Google Scholar 

  • Prabhudesai VR & Narayanaswamy S (1974) Organogenesis in tissue culture of certain Asclepiads. Z. Pflanzen. Physiol. 71: 181–185

    Google Scholar 

  • Pradhan C, Pattnaik Dwari M, Patnaik SN & Chand PK (1998) Efficient plant regeneration from cell suspension derived callus of East Indian rosewood (Dalbergia latifolia Roxb.). Plant Cell Rep. 18: 138–142

    Article  CAS  Google Scholar 

  • Rao PS, Narayanaswamy S & Benjamin BD (1970) Differentiation ex ovulo of embryos plantlets in stem tissue cultures of Tylophora indica. Physiol. Plant. 23: 140–144

    Article  Google Scholar 

  • Rao PVL & De DN (1987) Tissue culture propagation of tree legume Albizia lebbeck (L.) Benth. Plant Sci. 51: 263–267

    CAS  Google Scholar 

  • Ripley KP & Preece JE (1986) Micropropogation of Euphorbia lathyris L. Plant Cell Tiss. Org. Cult. 5: 213–218

    Article  CAS  Google Scholar 

  • Roy AT & De DN (1990) Tissue culture and plant regeneration from immature embryo explants of Calotropis gigantea (Linn.) R. Br. Plant Cell Tiss. Org. Cult. 20: 229–233

    CAS  Google Scholar 

  • Sekar T & Francis K (1998) Some plant species screened for energy, hydrocarbons and phytochemicals. Bioresource Tech. 65: 257–259

    Article  CAS  Google Scholar 

  • Smith MK (1983) In vitro propagation of Guayule (Parthenium argentatum Gray). Plant Sci. 31: 275–282

    Article  CAS  Google Scholar 

  • Tideman J & Hawker JS (1982) In vitro propagation of latex producing plants. Ann. Bot. 49: 273–279

    Google Scholar 

  • Wilson HM & Street HE (1975) The growth, anatomy and morphogenic potential of callus and cell suspension cultures of Hevea brasiliensis. Ann. Bot. 39: 671–682

    Google Scholar 

  • Wilson KJ & Mahlberg PG (1977) Investigation of laticifer differentiation in tissue cultures derived from Asclepias syriaca L. (Asclepiadacece). Am. J. Bot. 65: 98–109

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Applied Botany Section, Agricultural Engineering Department, Indian Institute of Technology, Kharagpur, West Bengal, 721 302, India

    Ashis T. Roy & Deepesh N. De

  2. Cell Biology Group, School of Plant Science, University of Tasmania, GPO Box 252-55, Hobart, TAS 7001, Australia

    Ashis T. Roy, Ashis T. Roy & Anthony Koutoulis

Authors
  1. Ashis T. Roy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anthony Koutoulis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Deepesh N. De
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ashis T. Roy.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roy, A.T., Koutoulis, A. & De, D.N. Cell suspension culture and plant regeneration in the latex-producing plant, Calotropis gigantea (Linn.) R. Br.. Plant Cell, Tissue and Organ Culture 63, 15–22 (2000). https://doi.org/10.1023/A:1006497220647

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006497220647

Search

Navigation