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Influence of in vitro growth conditions on photosynthetic competence and survival rate of Rehmannia glutinosa plantlets during acclimatization period

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Abstract

The photosynthetic responses of Rehmannia glutinosa grown under photoautotrophic or heterotrophic conditions in vitro were investigated after transfer to greenhouse conditions. In addition, the changes in carbohydrate content and survival rates of the plantlets were evaluated. During six days after transplantation, the photosynthetic rate declined and photoinhibitory impairments represented by decrease of Fv/Fm and chlorophyll content were observed regardless of environmental conditions in vitro. Excessive transpiration was observed in plantlets grown under heterotrophic conditions during that period. Fructose and glucose content of the plantlets grown under photoautotrophic conditions increased with time and reached almost the same level of field grown plants after day 15. Under heterotrophic conditions, in contrast, the content of these sugars decreased continuously during that period. It is suggested that high survival rate of plantlets grown under photoautotrophic conditions has to be attributed to improvement of photosynthetic competence by imposed high light intensity and CO2 concentration in vitro. The results strongly suggest that the control of transpiration during early stage after transplantation plays a key role in the acclimatization process, and photoautotrophic conditions could be a solution to solve the problems associated with transplantation stress.

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Author notes

  1. Jeong-Hoon Seon

    Present address: Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-ju, 361-763, South Korea

Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-ju, 361-763, South Korea

    Yong-Yi Cui, Kee-Yoeup Paek & Kee-Yoeup Paek

  2. Faculty of Horticulture, Chiba University, Matsudo, Chiba, 271, Japan

    Toyoki Kozai

Authors
  1. Jeong-Hoon Seon
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  2. Yong-Yi Cui
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  3. Toyoki Kozai
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  4. Kee-Yoeup Paek
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Correspondence to Kee-Yoeup Paek.

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Seon, JH., Cui, YY., Kozai, T. et al. Influence of in vitro growth conditions on photosynthetic competence and survival rate of Rehmannia glutinosa plantlets during acclimatization period. Plant Cell, Tissue and Organ Culture 61, 135–142 (2000). https://doi.org/10.1023/A:1006473223286

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