Abstract
A protocol for rapid shoot organogenesis from petiole explants of the ornamental aquatic plantNymphoides indica L. Thwaites O. Kuntze was developed for use in future mutation breeding and cultivar selection studies. Optimum culture conditions for shoot organogenesis were determined. Effects of factorial combinations of 2-iP, BA or kinetin (0–25 μM) in factorial combination with IAA or NAA (0–25 μM) were examined. On the basis of regeneration frequency (80%) and adventitious shoot number (11.5 shoots per explant), most efficient shoot organogenesis occurred on petiole explants cultured on a basal medium consisting of full-strength MS inorganic salts, 0.56 mM myo-inositol, 1.2 μM thiamine-HCl, 116.8 mM sucrose supplemented with 10 μM BA and 20 μM IAA and solidified with 0.8% TC agar. Formation of adventitious shoots by direct and indirect shoot organogenesis from the same explant was verified by histological sectioning. With the exception of variegated leaf production on a single adventitious shoot produced in the presence of 25 μM kinetin and 15 μM NAA, no visible phenotypic abnormalities were observedin vitro in any of the shoots generated. Solid achlorophyllous adventitious shoots were recovered following culture of this variegated leaf tissue. Plantlets were easily acclimatized toex vitro conditions.
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References
Bailey LH & Bailey EZ (1976) Hortus Third: a Concise Dictionary of Plants Cultivated in the United States and Canada. Macmillan, New York
Blakesley D & Lenton JR (1987) Cytokinin uptake and metabolism in relation to shoot multiplication in vitro. In: Jackson MB, Mantell SH & Blake J (eds) Advances in the Chemical Manipulation of Plant Tissue Cultures, No. 16 (pp. 87–99). British Plant Growth Working Group, Bristol
Broertjes C & Leffring L (1972) Mutation breeding of Kalanchoe. Euphytica 21: 415–423
Broertjes C & Van Harten AM (1978) Application of Mutation Breeding Methods in the Improvement of Vegetatively Propagated Crops. Elsevier Scientific Publishing Company, Amsterdam
Dore Swamy RD & Mohan Ram HY (1969) Studies on growth and flowering in axenic cultures of insectivorous plants. I. Seed germination and establishment of cultures of Utricularia inflexa. Phytomorphology 19: 363–371
Godmaire H & Nalewajko C (1986) Axenic culture of Myriophyllum spicatum L.: Importance to extracellular product estimates. Aquat. Bot. 26: 385–392
Harder R (1968) Axenische kultur von Aponogenton distachyus L. Planta 82: 193–199
Huang LC, Chang YH & Chang CL (1994) Rapid in vitro multiplication of the aquatic angiosperm, Anubias barteri var. undulata. Aquat. Bot. 47: 77–83
Ibrahim R, Mondelaers W & Debergh PC (1998) Effects of Xirradiation on adventitious bud regeneration from in vitro leaf explants of Rosa hybrida. Plant Cell Tiss. Org. Cult. 54: 37–44
Kakkar M & Mohan Ram HY (1986) Regeneration of whole plants from tissue cultures of the tropical aquatic legume, Neptunia oleracea. J. Plant Physiol. 126: 83–91
Kane ME & Albert LS (1989) Comparative root and shoot regeneration from juvenile and adult aerial leaf explants of variable-leaf milfoil. J. Aquat. Plant Manage. 27: 1–10
Kane ME, Davis GL, McConnell DB & Gargiulo JA (1999) In vitro propagation of Cryptocoryne wendtii. Aquat. Bot. 63: 197–202
Kane ME & Gilman EF (1991) In vitro propagation and assay systems for evaluating growth regulator effects on Myriophyllum species. J. Aquat. Plant Manage. 29: 29–32
Kane ME, Gilman EF & Jenks MA (1991) Regenerative capacity of Myriophyllum aquaticum cultured in vitro. J. Aquat. Plant Manage. 29: 102–109
Kane ME, Philman NL, Bartuska CA & McConnell DB (1993) Growth regulator effects on in vitro shoot regeneration of Crassula helmsii. J. Aquat. Plant Manage. 31: 59–64
Madsen JD (1985) The use of vegetative propagules for sterile cultures of aquatic macrophytes. Mich. Bot. 24: 169–174
Meyberg M (1988) Cytochemistry and ultrastructure of the mucilage secreting trichomes of Nymphoides peltata (Menyanthaceae). Ann. Bot. 62: 537–547
Mohan Ram HY & Kakkar M (1983) Role of tissue culture in the study of aquatic plants. Bull. Bot. Surv. India 25: 26–34
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Rao S & Mohan Ram HY (1981) Regeneration of whole plants from cultured root tips of Limnophila indica. Can. J. Bot. 59: 969–973
Sagawa Y & Kunisaki JT (1990) Micropropagation of floricultural crops. In: Ammirato PV, Evans DA, Sharp WR & Bajaj YPS (eds) Handbook of Plant Cell Culture, Vol. 5, Ornamental Species (pp 25–56). McGraw-Hill, New York
Sakai WS (1973) Simple method for differential staining of paraffin embedded plant material using toluidine blue O. Stain Tech. 48: 247–249
SAS Institute, Inc. (1985) SAS User's Guide: Statistics. SAS Institute, Inc, Cary, NC
Sculthorpe CD (1967) The Biology of Aquatic Vascular Plants. Edward Arnold, London
Sutton D (1994) Culture of water snowflake. Proc. Fla State Hort. Soc. 107: 409–413
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Jenks, M.A., Kane, M.E. & McConnell, D.B. Shoot organogenesis from petiole explants in the aquatic plant Nymphoides indica . Plant Cell, Tissue and Organ Culture 63, 1–8 (2000). https://doi.org/10.1023/A:1006471027372
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DOI: https://doi.org/10.1023/A:1006471027372