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Chlorophyll fluorescence transients of Photosystem II membrane particles as a tool for studying photosynthetic oxygen evolution

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Abstract

The rise of the chlorophyll fluorescence yield of Photosystem II (PS II) membranes as induced by high-intensity actinic light comprises only two distinct phases: (1) the initial O-J increase and (2) the subsequent J-P increase. Partial inhibition of the PS II donor side by heating or washing procedures which remove peripheral PS II proteins or cofactors of the oxygen-evolving complex results in decrease of magnitude and rate of the J-P phase. The rate constant of the J-P increase is directly proportional to the steady-state rate of oxygen evolution; complete suppression of the J-P phase corresponds to full inhibition. A characteristic dip after J-level is observed only in Tris-washed or severely heated PS II membranes; manganese release correlates with appearance of the dip after J-level as verified by EPR spectroscopy. Presence of stabilizing cosolutes (glycine betaine, sucrose) or addition of donor-side cofactors (bicarbonate, chloride, calcium) to PS II membranes before heating (47 °C, 5 min) diminishes J-P phase suppression and prevents dip appearance, whereas the addition after heating is without effect. In conclusion, analysis of chlorophyll fluorescence transients of PS II membranes is a potentially useful tool for investigations on photosynthetic oxygen evolution. A decreased rate of the J-P phase can be employed as a convenient indicator for partial inhibition of oxygen-evolution activity; the appearance of a dip after J-level is suggestive of manganese release.

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Author notes

  1. Pavel Pospíšil

    Present address: Department of Experimental Physics, Faculty of Science, Palacky University, Olomouc, Czech Republic

Authors and Affiliations

  1. FB Biologie/Botanik, Philipps-Universität, Lahnberge, D-35032, Marburg, Germany

    Pavel Pospíšil & Holger Dau

  2. FB Physik, Freie Universität Berlin, Arminallee 14, D-14195, Berlin, Germany

    Pavel Pospíšil

  3. FB Physik, Freie Universität Berlin, Arminallee 14, D-14195, Berlin, Germany

    Holger Dau

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  1. Pavel Pospíšil
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Correspondence to Holger Dau.

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Pospíšil, P., Dau, H. Chlorophyll fluorescence transients of Photosystem II membrane particles as a tool for studying photosynthetic oxygen evolution. Photosynthesis Research 65, 41–52 (2000). https://doi.org/10.1023/A:1006469809812

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