Skip to main content
SpringerLink
Log in

The diageotropica mutation alters auxin induction of a subset of the Aux/IAA gene family in tomato

  • Published:
Plant Molecular Biology Aims and scope Submit manuscript

Abstract

The diageotropica (dgt) mutation has been proposed to affect either auxin perception or responsiveness in tomato plants. It has previously been demonstrated that the expression of one member of the Aux/IAA family of auxin-regulated genes is reduced in dgt plants. Here, we report the cloning of ten new members of the tomato Aux/IAA family by PCR amplification based on conserved protein domains. All of the gene family members except one (LeIAA7) are expressed in etiolated tomato seedlings, although they demonstrate tissue specificity (e.g. increased expression in hypocotyls vs. roots) within the seedling. The wild-type auxin-response characteristics of the expression of these tomato LeIAA genes are similar to those previously described for Aux/IAA family members in Arabidopsis. In dgt seedlings, auxin stimulation of gene expression was reduced in only a subset of LeIAA genes (LeIAA5, 8, 10, and 11), with the greatest reduction associated with those genes with the strongest wild-type response to auxin. The remaining LeIAA genes tested exhibited essentially the same induction levels in response to the hormone in both dgt and wild-type hypocotyls. These results confirm that dgt plants can perceive auxin and suggest that a specific step in early auxin signal transduction is disrupted by the dgt mutation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Abel, S. and Theologis, A. 1995. A polymorphic bipartite motif signals nuclear targeting of early auxin-inducible proteins related to PS-IAA4 from pea (Pisum sativum). Plant J. 8: 87–96.

    Google Scholar 

  • Abel, S. and Theologis, A. 1996. Early genes and auxin action. Plant Physiol. 111: 9–17.

    Google Scholar 

  • Abel, S., Oeller, P.W. and Theologis, A. 1994. Early auxin-induced genes encode short-lived nuclear proteins. Proc. Natl. Acad. Sci. USA 91: 326–330.

    Google Scholar 

  • Abel, S., Nguyen, M.D. and Theologis, A. 1995. The PS-IAA4/5-like family of early auxin-inducible mRNAs in Arabidopsis thaliana. J. Mol. Biol. 251: 533–549.

    Google Scholar 

  • Ainley, W.M., Walker, J.C., Nagao, R.T. and Key, J.L. 1988. Sequence and characterization of two auxin-regulated genes from soybean. J. Biol. Chem. 263: 10658–10666.

    Google Scholar 

  • Ballas, N., Wong, L.-M., Ke, M. and Theologis, A. 1995. Two auxin-responsive domains interact positively to induce expression of the early indoleacetic acid-inducible gene PS-IAA4/5. Proc. Natl. Acad. Sci. USA 92: 3483–3487.

    Google Scholar 

  • Bowler, C., Neuhaus, G., Yamagata, H. and Chua, N.-H. 1994. Cyclic GMP and calcium mediate phytochrome phototransduction. Cell 77: 73–81.

    Google Scholar 

  • Chomczynski, P. and Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156–159.

    Google Scholar 

  • Cleland, R.E. 1995. Auxin and cell elongation. In: P.J. Davies (Ed.) Plant Hormones: Physiology, Biochemistry and Molecular Biology, Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 214–228.

    Google Scholar 

  • Conner, T.W., Goekjian, V.H., LaFayette, P.R. and Key, J.L. 1990. Structure and expression of two auxin-inducible genes from Arabidopsis. Plant Mol. Biol. 15: 623–632.

    Google Scholar 

  • Daniel, S.G., Rayle, D.L. and Cleland, R.E. 1989. Auxin physiology of the tomato mutant diageotropica. Plant Physiol. 91: 804–807.

    Google Scholar 

  • Felsenstein, J. 1989. PHYLIP: phylogeny inference package version 3.2. Cladistics 5: 164–166.

    Google Scholar 

  • Fleming, A.J., Mandel, T., Roth, I. and Kuhlemeier, C. 1993. The patterns of gene expression in the tomato shoot apical meristem. Plant Cell 5: 297–309.

    Google Scholar 

  • Fujino, D.W., Nissen, S.J., Jones, A.D., Burger, D.W. and Bradford, K.J. 1988. Quantification of indole-3-acetic acid in dark-grown seedlings of the diageotropica and epinastic mutants of tomato (Lycopersicon esculentum Mill.). Plant Physiol. 88: 780–784.

    Google Scholar 

  • Hashimoto, H. and Yamamoto, K.T. 1997. Three more members of the Aux/IAA gene family from mung bean (Vigna radiata) hypocotyl. Plant Physiol. 115: 315.

    Google Scholar 

  • Jackson, M.B. 1979. Is the diageotropic tomato ethylene deficient? Physiol. Plant 46: 347–351.

    Google Scholar 

  • Kelly, M.O. and Bradford, K.J. 1986. Insensitivity of the diageotropica tomato mutant to auxin. Plant Physiol. 82: 713–717.

    Google Scholar 

  • Kim, J., Harter, K. and Theologis, A. 1997. Protein-protein interactions among the Aux/IAA proteins. Proc. Natl. Acad. Sci. USA 94: 11786–11791.

    Google Scholar 

  • Lomax, T.L., Coenen, C., Gaiser, J.C., Hopkins, R., Rayle, D.L. and Rice, M.S. 1993. Auxin perception and the regulation of tomato growth and development. In: J.I. Yoder (Ed.) Molecular Biology of Tomato, Technomic Publishing Co., Lancaster, UK, pp. 129–138.

    Google Scholar 

  • Mito, N. and Bennett, A.B. 1995. The diageotropica mutation and synthetic auxins differentially affect the expression of auxinregulated genes in tomato. Plant Physiol. 109: 293–297.

    Google Scholar 

  • Muday, G.K., Lomax, T.L. and Rayle, D.L. 1995. Characterization of the growth and auxin physiology of roots of the tomato mutant diageotropica. Planta 195: 548–553.

    Google Scholar 

  • Nebenführ, A. and Lomax, T.L. 1998. Multiplex titration RT-PCR: a method for rapid determination of gene expression patterns for a large number of genes. Plant Mol. Biol. Rep. 16: 323–339.

    Google Scholar 

  • Neuhaus, G., Bowler, C., Kern, R. and Chua, N.-H. 1993. Calcium/calmodulin-dependent and-independent phytochrome signal transduction pathways. Cell 73: 937–952.

    Google Scholar 

  • Oeller, P.W., Keller, J.A., Parks, J.E., Silbert, J.E. and Theologis, A. 1993. Structural characterization of the early indoleacetic acidinducible genes, PS-IAA4/5 and PS-IAA6, of pea (Pisum sativum L.). J. Mol. Biol. 233: 789–798.

    Google Scholar 

  • Oono, Y., Chen, Q.G., Overvoorde, P.J., Köhler, C. and Theologis, A. 1998. age mutants of Arabidopsis exhibit altered auxin-regulated gene expression. Plant Cell 10: 1649–1662.

    Google Scholar 

  • Rice, M.S. and Lomax, T.L. 2000. The auxin-resistant diageotropica mutant of tomato responds to gravity via an auxin-mediated pathway. Planta, in press.

  • Rogers, S.O. and Bendich, A.J. 1994. Extraction of total cellular DNA from plants, algae and fungi. In: S.B. Gelvin and R.A. Schilperpoort (Eds.) Plant Molecular Biology Manual, Kluwer Academic Publishers, Dordrecht, Netherlands, pp. D1: 1–8.

    Google Scholar 

  • Rouse, D., Mackay, P., Stirnberg, P. and Leyser, O. 1998. Changes in auxin reponse from mutations in an AUX/IAA gene. Science 279: 1371–1373.

    Google Scholar 

  • Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425.

    Google Scholar 

  • Theologis, A., Huynh, T.V. and Davis, R.W. 1985. Rapid induction of specific mRNAs by auxin in pea epicotyl tissue. J. Mol. Biol. 183: 53–68.

    Google Scholar 

  • Thompson, J.D., Higgins, D.G. and Gibson, T.J. 1994. ClustalW: improving the sensitivity of progressive multiple sequence alignments through sequence weighting, position specific gap penalties and weight matrix choice. Nucl. Acids Res. 22: 4679–4680.

    Google Scholar 

  • Tian, Q. and Reed, J. 1999. Control of auxin-regulated root development by the Arabidopsis thaliana SHY2/IAA3 gene. Development 126: 711–721.

    Google Scholar 

  • Ulmasov, T., Hagen, G. and Guilfoyle, T.J. 1997a. ARF1, a transcription factor that binds to auxin response elements. Science 276: 1865–1868.

    Google Scholar 

  • Ulmasov, T., Murfett, J., Hagen, G. and Guilfoyle, T.J. 1997b. Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. Plant Cell 9: 1963–1971.

    Google Scholar 

  • Ulmasov, T., Hagen, G. and Guilfoyle, T.J. 1999. Dimerization and DNA binding of auxin response factors. Plant J. 19: 309–319.

    Google Scholar 

  • Yamamoto, K.T., Mori, H. and Imaseki, H. 1992. cDNA cloning of indole-3-acetic acid-regulated genes: Aux22 and SAUR from mung bean (Vigna radiata) hypocotyl tissue. Plant Cell Physiol. 33: 93–97.

    Google Scholar 

  • Zobel, R.W. 1974. Control of morphogenesis in the ethylenerequiring tomato mutant, diageotropica. Can. J. Bot. 52: 735–743.

    Google Scholar 

  • Zurek, D.M., Rayle, D.L., McMorris, T.C. and Clouse, S.D. 1994. Investigation of gene expression, growth kinetics, and wall extensibility during brassinosteroid-regulated stem elongation. Plant Physiol. 104: 505–513.

    Google Scholar 

Download references

Author information

Author notes

  1. Andreas Nebenführ

    Present address: MCD Biology, University of Colorado, Boulder, CO, 80309-0347, USA

Authors and Affiliations

  1. Department of Botany and Plant Pathology and Center for Gene Research and Biotechnology, Oregon State University, 2082, Cordley, Corvallis, OR, 97331-2902, USA

    TJ White & Terri L. Lomax

Authors
  1. Andreas Nebenführ
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. TJ White
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Terri L. Lomax
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nebenführ, A., White, T. & Lomax, T.L. The diageotropica mutation alters auxin induction of a subset of the Aux/IAA gene family in tomato. Plant Mol Biol 44, 73–84 (2000). https://doi.org/10.1023/A:1006437205596

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006437205596

Search

Navigation