Abstract
A method for the micropropagation of Lippia junelliana (Mold.) Tronc. from shoot tips or nodal segments was developed. Proliferating microshoot cultures were obtained by placing shoot tips or nodal segments on full strength Murashige and Skoog medium (MS) supplemented with 4.4 μM benzyladenine (BA) or 0.04 μM indolebutyric acid- (IBA) plus 4.4 μM BA. The rooting of shoots was better on full-strength MS medium without growth regulators. Rooted plantlets were successfully acclimatized to soil. The shoot cultures showed a lower essential oil accumulation in comparison with parent plants. Essential oil accumulation is closely related with growth and shows a negative correlation with shoot proliferation.
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Juliani, H.R., Koroch, A.R., Juliani, H.R. et al. Micropropagation of Lippia junelliana (Mold.) Tronc.. Plant Cell, Tissue and Organ Culture 59, 175–179 (1999). https://doi.org/10.1023/A:1006396531647
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DOI: https://doi.org/10.1023/A:1006396531647