Abstract
Murashige and Skoog (1962) medium supplemented with 1.0 to 4.5 μM of BA and 1.0 μM of NAA induced adventitious bud formation and shoot development in leaf explants of Roman Chamomile. A higher number of adventitious buds was observed at the proximal end of the explants. Plantlets were replicated and multiplied on MS medium supplemented with 2.25 μM of BA and 0.6 μM of IAA. Plantlets were rooted on MS medium supplemented with 0.5 μM of IBA and successfully weaned in vivo. The plants grew to maturity with high uniformity and no morphological signs of somaclonal variation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Biasio S (1997) Cultura de tecidos e micropropagação de Anthemis nobilis L. M.S. Thesis, University of Caxias do Sul
Debergh P, Aitken-Christie J, Cohen D, Grout B, von Arnold S, Zimmerman R& Ziv M (1992) Reconsideration of the term ‘vitrification’ as used in micropropagation. Plant Cell Tiss. Org. Cult. 30: 135–140
Evans DA (1989) Somaclonal variation – genetic basis and breeding applications. Trends Genet. 5: 46–50
Fauconnier ML, Jaziri M, Marlier M, Roggemans J, Wathelet JP, Lognay G, Severin M, Homés J& Shimomura K (1993) Essential oil production by Anthemis nobilis L. tissue culture. J. Plant Physiol. 141: 759–761
Fauconnier ML, Jaziri M, Homés J, Shimomura K& Marlier M (1996) Anthemis nobilis L. (Roman Chamomile): In vitro culture, micropropagation and the production of essential oils. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol 37, Medical and Aromatic Plants IX, (pp. 16–37)
Leug AY (1980) Encyclopedia of Common Natural Ingredients: Used in Food, Drugs, and Cosmetics. John Wiley&Sons, New York
Murashige T& Skoog FS (1962) A revised medium for rapid growth and biossay with tobacco tissue culture. Physiol. Plant. 15: 473–497
Reynolds JEF (ed.) (1982) Martindale: The Extra Pharmacopeia. The Pharmaceutical Press, London
Stefaan P, Werbrouck O& Debergh PC (1994) Applied aspects of plant regeneration. In: Dixon RA& Gonzales RA (eds) Plant Cell Culture: A Practical Approach (pp. 127–145). Oxford University Press, Oxford, NY
Ziv M (1991) Vitrification: morphological and physiological disorders of in vitro plants. In: Debergh PC& Zimmerman RH (eds) Micropropagation – Technology and Application (pp. 45–69). Kluwer Academic Publishers, Dordrecht
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Echeverrigaray, S., Fracaro, F., Andrade, L. et al. In vitro shoot regeneration from leaf explants of Roman Chamomile. Plant Cell, Tissue and Organ Culture 60, 1–4 (2000). https://doi.org/10.1023/A:1006368210670
Issue Date:
DOI: https://doi.org/10.1023/A:1006368210670