Abstract
Sporophyte regeneration through green globular bodies (GGB) is affected by the supply of growth regulators as well as by the source of the donor explant. Rhizome, frond, petiole or root tip explants from juvenile sporophytes of Polypodium cambricum were cultured on media containing N6-benzyladenine (BA) or Kinetin alone or in combination with α-naphthaleneacetic acid (NAA). GGB production took place even in growth regulator-free medium. Addition of BA into the culture medium was very effective to induce formation of GGB in rhizome explants. Root tip explants exhibited the lowest organogenic capacity. Homogenization of BA-pretreated frond or rhizome was a powerful system to improve fern propagation.
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Bertrand, A., Albuerne, M., Fernández, H. et al. In vitro organogenesis of Polypodium cambricum. Plant Cell, Tissue and Organ Culture 57, 65–69 (1999). https://doi.org/10.1023/A:1006348628114
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DOI: https://doi.org/10.1023/A:1006348628114