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Isolation and characterization of the wheat prolyl isomerase FK506-binding protein (FKBP) 73 promoter

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Abstract

The wheat FK506-binding protein (FKBP) 73 is a member of the peptidyl prolyl cis-trans isomerase gene family, which catalyses the interconversion between the cis and trans forms of the peptide bond preceding proline residues in proteins. A 3.5 kb sequence 5′ upstream of the ATG codon of the wheat FKBP73 was isolated from a wheat genomic library, and characterized by deletion analysis and transient expression in wheat embryos. The 1517 bp fragment is referred to as the full promoter due to the maximal activity of the fused luciferase reporter gene. Sequence analysis revealed the presence of three abscisic acid (ABA)-responsive elements (ABREs) proximal to coupling elements (CE1-like), a putative lectin box, two putative binding sites for the myb transcription factor and a 36 bp fragment which exhibits 100% identity to the pSau3A9 clone located in the centromeric region of wheat chromosomes. In a transient expression assay the promoter preserved the tissue specificity described in vivo, namely it is expressed only in germinating embryos and young shoots. The promoter was induced 1.9-fold by ABA, the minimal promoter was designated at −221 and the TATA box located at −137. The inducibility by ABA and the expression during germination may indicate that FKBP73 belongs to the group of genes induced by ABA upon germination.

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References

  • Alber, M.L. and Scandalios, J.G. 1993. Isolation and characterization of a genomic sequence encoding the maize Cat3 catalase gene. Plant Mol. Biol. 22: 1031–1038.

    Google Scholar 

  • Aviezer, K., Kurek, I., Erel, N., Blecher, O., Devos, K. and Breiman, A. 1998. Studies on the expression of the wheat prolyl isomerase FKBP73 during plant development. Plant Sci. 139: 149–158.

    Google Scholar 

  • Baker, J., Steele, C., Dure, L. III. 1988. Sequence and characterization of 5 Lea proteins and their genes from cotton. Plant Mol. Biol. 11: 277–291.

    Google Scholar 

  • Blecher, O., Erel, N., Callebaut, I., Aviezer, K. and Breiman, A. 1996. A novel wheat peptidyl-prolyl cis trans isomerase: cDNA isolation, structure, enzymatic activity and expression. Plant Mol. Biol. 32: 493–504.

    Google Scholar 

  • Breiman, A., Fawcett, T.W., Ghirardi, M.L. and Mattoo, A.K. 1992. Plant organelles contain distinct peptidyl-prolyl-cis-transisomerases. J. Biol. Chem. 267: 21293–21296.

    Google Scholar 

  • Cheng, J.-C., Seeley, K.A., Goupil, P. and Sung, Z.R. 1996. Expression of DC8 is associated but not dependent on embryogenesis. Plant Mol. Biol. 31: 127–141.

    Google Scholar 

  • Christensen, A.H., Sharrock, R.A. and Quail, P.H. 1992. Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation. Plant Mol. Biol. 18: 675–689.

    Google Scholar 

  • Dolinski, K. and Heitman, E. 1997. Peptidyl-prolyl isomerases: an overview of the cyclophilin FKBP and parvulin families. In:M.J. Gething (Ed.), Guidebook to Molecular Chaperones and Protein Folding Catalysis, Sambrook & Tooze Guidebook Series, Oxford University Press, Oxford, pp. 359–369.

    Google Scholar 

  • Faure, J.-D., Gingerich, D. and Howell, S.H. 1998. An arabidopsis immunophilin, AtFKBP12, binds to AtFIP37 (FKBP interacting protein) in an interaction that is disrupted by FK506. Plant J. 15: 783–789.

    Google Scholar 

  • Foster, R., Izawa, T. and Chua, N.-H. 1994. Plant bZip proteins gather at ACGT elements. FASEB J. 8: 192–200.

    Google Scholar 

  • Fulgosi, H., Vener, A.V., Altschmied, L., Herrmann, R.G, and Andersson, B. 1998. A novel multi-functional chloroplast protein: identification of a 40 kDa immunophilin-like protein located in the thylakoid lumen. EMBO J. 17: 1577–1587.

    Google Scholar 

  • Galat, A. and Metcalfe, S. 1995. Peptidyl-prolyl cis-trans isomerases. Prog. Biophys. Mol. Biol. 63: 67–118.

    Google Scholar 

  • Gerlach, W.L. and Bedbrook, J.R. 1979. Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucl. Acids Res. 7: 1869–1885

    Google Scholar 

  • Giuliano, G., Pichersky, E., Malik, V.S., Timko, M.P., Scolnik, P.A. and Cashmore, A.R. 1988. An evolutionarily conserved protein binding sequence upstream of a plant regulated gene. Proc. Natl. Acad. Sci. USA 85: 7089–7093.

    Google Scholar 

  • Guiltinan, M.J., Marcotte, W.R. and Quatrano, R.S. 1990. A plant leucine zipper protein that recognises an abscisic acid response element. Science 250: 267–270.

    Google Scholar 

  • Hattori, T., Terada, T. and Hamasuna, S. 1995. Regulation of the Osem gene by abscisic acid and the transcription factor VP1: analysis of the cis-acting promoter elements required for regulation by abscisic acid and VP1. Plant J. 7: 913–925.

    Google Scholar 

  • Higo, K. and Higo, H. 1996. Cloning and characterization of the rice CatA catalase gene, a homologue of the maize Cat3 gene. Plant Mol. Biol. 30: 505–521.

    Google Scholar 

  • Hill, A., Nantel, A., Rock, C.D. and Quatrano, R.S. 1996. A conserved domain of the viviparous-1 gene product enhances the DNA binding activity of the bZIP protein emBP-1 and other transcription factors. J. Biol. Chem. 271: 3366–3374.

    Google Scholar 

  • Holappa, L.D. and Walker-Simmons, M.K. 1995. The wheat abscisic acid-responsive protein kinase mRNA, PKABA1, is upregulated by dehydration, cold temperature and osmotic stress. Plant Physiol. 108: 1203–210.

    Google Scholar 

  • Hong, B., Barg, R. and Ho, T.D. 1992. Developmental and organspecific expression of an ABA-and stress-induced protein in barley. Plant Mol. Biol. 18: 663–674.

    Google Scholar 

  • Hueros, G., Rahfeld, J., Salamini, F. and Thompson, R. 1998. A maize FK506-sensitive immunophilin, mzFKBP-66 is a peptidyl proline cis-trans-isomerase that interacts with calmodulin and a 36-kDa cytoplasmic protein. Planta 205: 121–131.

    Google Scholar 

  • Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387–405.

    Google Scholar 

  • Jiang, J., Nasuda, S., Dong, F., Scherrer, C.W., Woo, S.-S., Wing, R.A., Gill, B.S. and Ward, D.C. 1996. A conserved repetitive DNA element located in the centromers of cereal chromosomes. Proc. Natl. Acad. Sci. USA 93: 14210–14213.

    Google Scholar 

  • Kurek, I., Herman, E., Erel, N., Aviezer, K. and Breiman, A. 1999. The wheat peptidyl prolyl cis trans isomerase, FKBP77, is heat induced and developmentally regulated. Plant Physiol. 119: 693–704.

    Google Scholar 

  • Lane, B.G. 1991. Cellular desiccation and hydration: developmentally regulated proteins, and the maturation and germination of seed embryos. FASEB J. 5: 2893–2900.

    Google Scholar 

  • Larkin, J.C., Oppenheimer, D.G., Pollock, S. and Marks, M.D. 1993. Arabidopsis GLABROUS1 gene requires downstream sequences for function. Plant Cell 5: 1739–1748.

    Google Scholar 

  • Liu, Z.-B., Ulmasov, T., Shi, X., Hagen, G. and Guilfoyle, T.J. 1994. Soybean GH3 promoter contains multiple auxin-inducible elements. Plant Cell 6: 645–657.

    Google Scholar 

  • Lonsdale, D.M., Moisan, L.J. and Harvey, A.J. 1998. The effect of altered codon usage on luciferase activity in tobacco, maize and wheat. Plant Cell Rep. 17: 396–399.

    Google Scholar 

  • Luan, S., Kudla, J., Gruissem, W. and Schreiber, S.L. 1996. Molecular characterisation of a FKBP-type immunophilin from higher plants. Proc. Natl. Acad. Sci. USA 93: 6964–6969.

    Google Scholar 

  • Marcotte, W.R., Russell, S.H. and Quatrano, R.S. 1989. Abscisic acid-responsive sequences from the Em gene of wheat. Plant Cell 1: 969–976.

    Google Scholar 

  • Martin, C. and Paz-Ares, J. 1997. MYB transcription factors in plants. Trends Genet. 13: 67–73.

    Google Scholar 

  • Mason, H.S. and Mullet, J.E. 1990. Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding and jasmonic acid. Plant Cell 2: 569–579.

    Google Scholar 

  • Mundy, J. and Chua, N.-H. 1988. Abscisic acid and water stress induce the expression of a novel rice gene. EMBO J. 7: 2279–2286.

    Google Scholar 

  • Mundy, J., Yamaguchi-Shinozaki, K. and Chua, N.-H. 1990. Nuclear proteins bind conserved elements in the abscisic acid responsive promoter of a rice Rab gene. Proc. Natl. Acad. Sci. USA 87: 1406–1410.

    Google Scholar 

  • Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497.

    Google Scholar 

  • Shen, Q. and Ho, T.-H.D. 1995. Functional dissection of an abscisic acid (ABA)-inducible gene reveals two independent ABAresponsive complexes each containing a G-box and a novel cis-acting element. Plant Cell 7: 295–307.

    Google Scholar 

  • Shen, Q., Uknes, S.J. and Ho, T.-H.D. 1993. Hormone response complex of a novel abscisic acid and cycloheximide inducible barley gene. J. Biol. Chem. 268: 23652–23660.

    Google Scholar 

  • Shen, Q., Zang, P. and Ho, T.-H.D. 1996. Modular nature of abscisic acid (ABA) response complex: composite promoter units that are necessary and sufficient for ABA induction of gene expression in barley. Plant Cell 8: 1107–1119.

    Google Scholar 

  • Straub, P.F., Shen, Q. and Ho, T.-H.D. 1994. Structure and promoter analysis of an ABA-and stress-regulated barley gene, HVA1. Plant Mol. Biol. 26: 617–630.

    Google Scholar 

  • Tebbutt, S.J. and Lonsdale, D.M. 1995. Deletion analysis of a tobacco pollen-specific polygalacturonase promoter. Sex. Plant Reprod. 8: 242–246.

    Google Scholar 

  • Vittorioso, P., Cowling, R., Faure, J.D., Caboche, M. and Bellini, C. 1998. Mutation in the Arabidopsis pasticcino 1 gene, a new FKBP-like protein, has a dramatic effect on plant development. Mol. Cell Biol. 18: 3034–3043.

    Google Scholar 

  • Vucich, V.A. and Gasser, C.S. 1996. Novel structure of a high molecular weight FK506 binding protein from Arabidopsis thaliana. Mol. Gen. Genet. 252: 510–517.

    Google Scholar 

  • Xu, Q., Liang, S., Kudla, J. and Luan, S. 1998. Molecular characterization of a plant FKBP12 that does not mediate action of FK506 and rapamycin. Plant J. 15: 511–519.

    Google Scholar 

  • Yamaguchi-Shinozaki, K. and Shinozaki, K. 1994. A novel cisacting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6: 251–264.

    Google Scholar 

  • Yamaguchi-Shinozaki, K., Mundy, J. and Chua, N.-H. 1989. Four tightly linked rab differentially expressed in rice. Plant Mol. Biol. 14: 29–39.

    Google Scholar 

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Authors and Affiliations

  1. George S. Wise Faculty of Life Sciences, Department of Plant Sciences, Tel Aviv University, Tel Aviv, Israel, 69978

    Isaac Kurek & Adina Breiman

  2. Cambridge Laboratory, John Innes Centre, Colney Lane, Norwich, Norfolk, NR4 7UJ, UK

    Alison J. Harvey & David M. Lonsdale

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  1. Isaac Kurek
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  2. Alison J. Harvey
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  3. David M. Lonsdale
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  4. Adina Breiman
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Kurek, I., Harvey, A.J., Lonsdale, D.M. et al. Isolation and characterization of the wheat prolyl isomerase FK506-binding protein (FKBP) 73 promoter. Plant Mol Biol 42, 489–497 (2000). https://doi.org/10.1023/A:1006345411056

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