Abstract
Embryonic axes of Juglans regia werecultured in vitro on Murashige and Skoog mediumsupplemented with different auxin/cytokinin ratios.2,4D, NAA or IBA at 1, 2, 4, 8 mgl-1, alone orcombined with Kn 0.3 mgl-1 induced callus whichshowed browning. NAA and IBA allowed organ developmentwhich was inhibited by 2,4-D. Cell suspensions fromNAA-or IBA induced callus had embryogenic capacitywhen cultured with NAA or IBA, showing a heterogeneouscell population composed of giant, elongated, andmeristematic cells. Those cultured with IBA dividedfollowing embryogenic patterns and cell aggregateswhich were associated with the earliest steps ofembryoid formation. Cell suspension from 2,4-D inducedcallus, revealed homogeneous cell populations whichwere very vacuolated with no apparent differentiation.Axillary bud proliferation induced by BA at 0.1, 1 or5 mgl-1 was affected by both the physical stateof the culture medium and the time period in contactwith the growth regulator. The culture of embryonicaxes for one week in MS liquid medium with BA5 mgl-1 favoured proliferation as well as buddevelopment.
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Fernández, H., Pérez, C. & Sánchez-Tamés, R. Modulation of the morphogenic potential of the embryonic axis of Juglans regia by cultural conditions. Plant Growth Regulation 30, 125–131 (2000). https://doi.org/10.1023/A:1006329111046
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DOI: https://doi.org/10.1023/A:1006329111046