Abstract
This study is the first to demonstrate that a foreign DNA can be delivered into cells of facultative halophyte crassulacean acid metabolism (CAM) plant, Mesembryanthemum crystallinum L. with Agrobacterium tumefaciens. This plant can be induced to change from C3 to CAM by drought and various stresses, and is a good model to study the environment stress on metabolism not only at whole plant but also at cell level. The β-glucuronidase (GUS) and kanamycin (Km) resistance genes were introduced into this plant. The average successful rate of transformation was about 54.5% with root tissue or 53.0% with hypocotyl tissue. Based on the resistance to Km, polymerase chain reaction (PCR) detection and GUS expression, transformation with Agrobacterium tumefaciens was successful for introducing foreign genes into M. crystallinum.
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References
Adams P, Thomas JC, Vernon DM, Bohnert HJ & Jensen RG (1992) Distinct cellular and organismic responses to salt stress. Plant Cell Physiol. 33: 1215–1223
Bohnert HJ, Ostrem JA, Cushman JC, Michalowski CB, Rickers J, Meyer G, DeRocher EJ, Vernon DM, Krueger M, Vazquez-Moreno L, Velton J, Höfner R & Schmitt JM (1988) Mesembryanthemum crystallinum, a higher plant model for the study of environmentally induced changes in gene expression. Plant Mol. Biol. Rep. 6: 10–28
Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387–405
Murray M & Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8: 4321–4325
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497
Thomas JC, DeArmond RL & Bohnert HJ (1992) Influence of NaCl on growth, proline, and phosphoenolpyruvate carboxylase levels in Mesembryanthemum crystallinum suspension cultures. Plant Physiol. 98: 626–631
Treichel S, Hettfleisch H, Eilhardt S, Faist K & Kluge M (1988) A possible induction of CAM by NaCl-stress in heterotrophic cell suspension cultures of Mesembryanthemum crystallinum. J. Plant Physiol. 133: 419–424
Willenbrink ME & Husemann W (1995) Photoautotrophic cell suspension cultures from Mesembryanthemum crystallinum and their response to salt stress. Bot. Acta 108: 497–504
Winter K (1985) Crassulacean acid metabolism. In: Photosynthetic Mechanisms and the Environment (pp 329–387). Elsevier, Amsterdam
Yen HE, Grimes HD & Edwards GE (1995) The effects of high salinity, water-deficit, and abscisic acid on phosphoenolpyruvate carboxylase activity and proline accumulation in Mesembryanthemum crystallinum cell cultures. J. Plant Physiol. 145: 557–564
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Ishimaru, K. Transformation of a CAM plant, the facultative halophyte Mesembryanthemum crystallinum by Agrobacterium tumefaciens. Plant Cell, Tissue and Organ Culture 57, 61–63 (1999). https://doi.org/10.1023/A:1006225212298
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DOI: https://doi.org/10.1023/A:1006225212298