Abstract
Effects of appling exogenous jasmonic acid (JA) on the germination of Dioscorea alata L. microtubers were examined on Murashige and Skoog (MS) medium. Microtuber germination was promoted by JA (0.1 and 1 μM) supplemented to the culture medium but higher concentrations (30 and 100 μM) completely inhibited germination. When these inhibited microtubers were transferred to hormone-free medium, germination resumed.
After transfer to greenhouse conditions, almost all plants (95%) from tubers previously cultivated on MS medium with 100 μM JA survived and all acclimatized plants had produced tubers after 8 months. It is concluded that depending on JA concentration, both the germination and dormancy processes in D. alata microtubers were affected. The release from dormancy is easily obtained by transferring dormant microtubers to hormone-free medium.
Similar content being viewed by others
References
Degras L, Arnolin R, Poitout A and Suard C (1977) Quelques aspects de la biologie des ignames (Dioscorea spp). Les ignames et leur culture. Ann Amélior Plantes 27: 1–23
Deliu C, Tamas M, Dobrota C and Munteanu-Deliu C (1992) Kinetics of cellular growth and diosgenin biosynthesis in Dioscorea caucasica cell suspensions, cultured in batch system. Plant Sci 85: 99–105
Demeaux M and Vivier P (1984) Méthodes modernes de conservation des ignames. Agronomie Tropicale 39: 186–191
Ishida BK (1988) Improved diosgenin production in Dioscorea deltoidea cell cultures by immobilization in polyurethane foam. Plant Cell Rep 7: 270–273
Kefi S, Read PE, Pavlista A and Kachman SD (1995) The effects of different concentrations of gibberellic acid (GA3) and kinetin on in vitro tuberization of potato (Solanum tuberosum L.). HortScience 30: 752
Koda Y and Kikuta Y (1991) Possible involvement of jasmonic acid in tuberization of yam plants. Plant Cell Physiol 32: 629–633
Koda Y, kikuta Y, Tazaki H, Tsujino Y, Sakamura S and Yoshihara T (1991) Potato tuber-inducing activities of jasmonic acid and related compounds. Phytochemistry 30: 1435–1438
Lacointe A and Zinsou C (1987) Croissance et developpement au champ de l'igname (Dioscorea alata L) à partir des plants produits par culture in vitro. Agronomie 7: 331–338
Mitchell SA, Asemota HN and Ahmad MH (1995) Effects of explant source, culture medium strength and growth regulators on the in-vitro propagation of three Jamaican yams (Dioscorea cayenensis, D trifida and D rotundata). J Sci Food Agric 67: 173–180
Murashige T and Skoog F (1962) A revised medium for rapid growth and BIO B assays with tobacco tissue cultures. Physiol Plant 15: 473–497
Nkounkou JS (1993) Etude taxonomique et phytogeographique des Dioscoreaceae de l'Afrique centrale (Congo, Zaire, Rwanda, Burundi). Thèse de doctorat, Université Libre de Bruxelles, Belgique
Okagami N (1978) Dormancy in Dioscorea: Sprouting promotion by inhibitors of protein synthesis in bulbils and rhizomes. Plant Cell Physiol 19: 221–227
Okagami N and Tanno N (1993) Gibberellic acid-induced prolongation of the dormancy in tubers or rhizomes of several species of East Asian Dioscorea. Plant Growth Regul 12: 119–123
Okagami N, Tanno N, Kawai M, Hiratsuka A, Satoh K-I and Keisuke T (1997) Expansion of trait of gibberellin-induced dormancy in the tertiary relict species of the genus Dioscorea (Dioscoreaceae). Plant Growth Regul 22: 137–140
Passam HC (1982) Dormancy of yams in relation to storage. In: Miege J and Lyonga SN (eds) Yams/Ignames, pp 285–293. Clarendon Press Oxford
Pelacho AM and Mingo-Castel AM (1991) Jasmonic acid induces tuberization of potato stolons cultured in vitro. Plant Physiol 97: 1253–1255
Ravi V and Aked J (1996) Review on tropical root and tubers crops. II. Physiological disorders in freshly stored roots and tubers. Crit Rev Food Sci Nutr 36: 711–731
Trouslot MF (1985) Analyse de la croissance et morphogenèse de l'igname Dioscorea complexe D. cayenensis-D. rotundata. ORSTOM, Paris
Vandenput R (ed.) (1981) Les principales cultures en Afrique centrale. Bruxelles
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bazabakana, R., Fauconnier, ML., Diallo, B. et al. Control of Dioscorea alata microtuber dormancy and germination by jasmonic acid. Plant Growth Regulation 27, 113–117 (1999). https://doi.org/10.1023/A:1006199432339
Issue Date:
DOI: https://doi.org/10.1023/A:1006199432339