Abstract
A new protocol was established for sugarcane cv. C-1051-73 shoot formation in a temporary immersion system. The two-step protocol involves shoot formation in 50 ml of culture medium per explant and 1.0 mg l-1 paclobutrazol for 30 days followed by shoot elongation by exposure to 1.0 mg l-1 gibberellic acid for 15 days. The multiplication rate was doubled in comparison with the conventional micropropagation protocol (Jiménez et al., 1995) and the cost has been reduced by 46%. Three additional sugarcane varieties have been micropropagated according to this new protocol and results are comparable. Temporary immersion-derived plants have also been compared with conventionally propagated plants in sugarcane fields for more than 9 months and their agricultural indicators of performance are similar.
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Lorenzo, J.C., González, B.L., Escalona, M. et al. Sugarcane shoot formation in an improved temporary immersion system. Plant Cell, Tissue and Organ Culture 54, 197–200 (1998). https://doi.org/10.1023/A:1006168700556
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DOI: https://doi.org/10.1023/A:1006168700556