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Identification of genes specifically expressed in cauliflower reproductive meristems. Molecular characterization of BoREM1

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Abstract

Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts.

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Author notes

  1. José Manuel Franco-Zorrilla

    Present address: Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología, Campus de la, Universidad Autónoma de Madrid, Cantoblanco, 28049, Madrid, Spain

Authors and Affiliations

  1. Departamento de Mejora Genética y Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra de La Coruña, km7, 28040, Madrid, Spain

    Begoña Fernández-calvín, Julio Salinas & José Miguel martínez-zapater

  2. Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-CSIC, Camino de Vera s/n, 46022, Valencia, Spain

    Francisco Madueño

  3. Department of Bioscience, Salem-Teikyo University, 223 W. Main, St., Salem, WV, 26426, USA

    Marilyn Cruz-Alvarez

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  1. José Manuel Franco-Zorrilla
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Franco-Zorrilla, J.M., Fernández-calvín, B., Madueño, F. et al. Identification of genes specifically expressed in cauliflower reproductive meristems. Molecular characterization of BoREM1. Plant Mol Biol 39, 427–436 (1999). https://doi.org/10.1023/A:1006130629100

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