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Conserved Ser residues in the basic region of the bZIP-type transcription factor HBP-1a(17): importance in DNA binding and possible targets for phosphorylation

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Abstract

HBP-1a(17) is representative of a group of plant bZIP-type transcription factors which includes HBP-1a proteins and G-box-binding factors. We found kinase activity in wheat nuclear extract that phosphorylated HBP-1a(17). Experiments using recombinant HBP-1a(17) derivatives as substrates revealed that all three of the Ser residues in the basic region, Ser-261, Ser-265, and Ser-269, were phosphorylated in a Ca2+-stimulated manner. DNA-binding analysis of mutants with a Ser-to-Glu change, prepared to mimic the phosphorylated proteins, indicated that introduction of a negative charge at position 265 or 269 prevents HBP-1a(17) from binding DNA not only in the homodimer of mutants but also in heterodimers with a wild-type protein. It is therefore suggested that the phosphorylation regulates the function of HBP-1a(17) at least at the level of DNA binding. Since Ser-265 and Ser-269 are highly conserved among the plant bZIP-type factors known to date, a common Ca2+-mediated regulatory mechanism may exert an effect on the bZIP-type factors through phosphorylation of these conserved Ser residues.

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Author notes

  1. Maki Minami

    Present address: Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo, 113, Japan

  2. Masahiro Okanami

    Present address: Nara Institute of Science and Technology, Ikoma, Nara, 630-01, Japan

Authors and Affiliations

  1. Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto, 606-01, Japan

    Tetsuo Meshi, Ichiro Moda & Masaki Iwabuchi

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Meshi, T., Moda, I., Minami, M. et al. Conserved Ser residues in the basic region of the bZIP-type transcription factor HBP-1a(17): importance in DNA binding and possible targets for phosphorylation. Plant Mol Biol 36, 125–136 (1998). https://doi.org/10.1023/A:1005934332530

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