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Cell suspension, isolation and culture of protoplasts of Allium cepa

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Abstract

Tissue culture techniques involving cell suspension, protoplast fusion and culture in the genus Allium are different as Allium is recalcitrant due to the biological peculiarity of the genus. A procedure is described for the establishment of a regenerable suspension culture and for the isolation and culture of protoplasts of Allium cepa.

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Authors and Affiliations

  1. Applied Genetics and Biotechnology Division, Faculty of Agriculture, Miyazaki University, Gakuen Kibanadai Nishi 1-1, Miyazaki, 889-21, Japan

    M. A. Karim & T. Adachi

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  1. M. A. Karim
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  2. T. Adachi
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Karim, M.A., Adachi, T. Cell suspension, isolation and culture of protoplasts of Allium cepa. Plant Cell, Tissue and Organ Culture 51, 43–47 (1997). https://doi.org/10.1023/A:1005886732424

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  • DOI: https://doi.org/10.1023/A:1005886732424

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