Abstract
Six wheat genotypes (three varieties and three whole chromosome substitution lines) were used to analyse the relationship between the initiation of green spots and plant regeneration under short-term tissue culture conditions. The highest percentage of green spot initiation was observed after one week culture on maintenance medium (M-Med.). The calluses producing green spots at the end of the first and second week on M-Med. were highest in regeneration frequency compared to late green spot producing ones. A significant positive correlation between green spot initiation and plantlet regeneration was observed for calluses showing green spots within the first two weeks on M-Med. and was decreased for calluses producing green spots afterwards. The results suggest that by selecting only calluses producing early green spots the experimental efficiency will be increased.
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Ben Amer, I.M., Börner, A. The relationship between green spot initiation and plantlet regeneration of wheat callus grown under short-term conditions. Plant Cell, Tissue and Organ Culture 50, 67–69 (1997). https://doi.org/10.1023/A:1005855912655
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DOI: https://doi.org/10.1023/A:1005855912655