Summary
The influence of genes affecting the ‘plant growth habit’ in wheat (Rht8 and Ppd1) and rye (ct1 and ct2) on tissue culture response was studied using immature embryos. Whereas the semi-dwarfing gene Rht8 seems to promote only a minor effect, the day-length sensitive allele ppd1 determined a major increase in callus growth and regeneration ability. With regards to their tissue culture efficiency, the four alleles studied could be ranked as follows: ppd1>Rht8>rht8>Ppd1.
In contrast to wheat, the GA insensitive semi-dwarfing genes of rye (ct1 and ct2) appear not to influence in vitro response.
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Ben Amer, I.M., Worland, A.J. & Börner, A. In vitro culture variation of wheat and rye caused by genes affecting plant growth habit in vivo . Euphytica 61, 233–240 (1991). https://doi.org/10.1007/BF00039663
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DOI: https://doi.org/10.1007/BF00039663