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A new Arabidopsis nucleic-acid-binding protein gene is highly expressed in dividing cells during development

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Abstract

An Arabidopsis thaliana cDNA encoding a new RNA-binding protein (RBP37) was cloned from a silique cDNA library. The predicted amino acid sequence corresponds to a RBP containing two RNA recognition motifs (RRM) and a basic domain. An affinity for nucleic acids was confirmed in binding assays using in vitro synthesised AtRBP37 protein. In situ hybridisation experiments on sections of flowers and siliques showed expression only in growing organs: gynoecium, petals, filaments and during early-embryogenesis expression is located in the embryo proper and the suspensor up to late heart stage. Expression is not detected in the embryo during maturation.This results suggests an expression pattern correlated with dividing cells.

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References

  1. Brand SF, Pichoff S, Noselli S, Bourbon HM: Novel Drosophila melanogastergenes encoding RRM-type RNA-binding proteins identified by a degenerate PCR strategy. Gene 154: 187-192 (1995).

    Google Scholar 

  2. Burd CG, Dreyfuss G: Conserved structures and diversity of functions of RNA-binding proteins. Science 265: 615-621 (1994).

    Google Scholar 

  3. Devic M, Hecht V, Berger C, Lindsey K, Delseny M, Gallois P: Assessment of promoter trap as a tool to study zygotic embryogenesis in Arabidopsis thaliana. C R Acad Sci Paris, Sciences de la Vie/Life sciences 318: 121-128 (1995).

    Google Scholar 

  4. Ferreira PCG, Hemerly AS, de Almeida Engler JA, van Montagu M, Engler G, Inze D: Developmental expression of Arabidopsiscyclin gene cycAt. Plant Cell 6: 1763-1774 (1994).

    Google Scholar 

  5. Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM: Isolation of the Arabidopsis ABI3gene by positional cloning. Plant Cell 4: 1251-1261 (1992).

    Google Scholar 

  6. Hirose T, Sugita M, Sugiura M: cDNA structure, expression and nucleic acid-binding properties of three RNA-binding proteins in tobacco: occurence of tissue-specific alternative splicing. Nucl Acids Res 21: 3981-3987 (1993).

    Google Scholar 

  7. Joshi CP: An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucl Acids Res 15: 6643-6653 (1987).

    Google Scholar 

  8. Jürgens G, Mayer U: Arabidopsis. In: Bard J (ed) Embryos. Colour Atlas of Development, pp. 7-21. Wolf publications, London (1994).

    Google Scholar 

  9. Kim YJ, Baker BS: The Drosophilagene RBP9encodes a protein that is a member of a conserved group of putative RNA binding proteins that are nervous system-specific in both flies and humans. J Neurosci 13: 1045-1056 (1993).

    Google Scholar 

  10. Marks C: DNA Strider: a C program for fast analysis of DNA and protein sequences on the Apple Macintosh family of computers. Nucl Acids Res 16: 1829-1836 (1988).

    Google Scholar 

  11. Martinez MC, Jorgensen JO, Lawton MA, Lamb CJ, Doerner PW: Spatial pattern of cdc2expression in relation to meristem activity and cell proliferation during plant development. Proc Natl Acad Sci USA 89: 7360-7364 (1992).

    Google Scholar 

  12. Smyth DR, Bowman JL, Meyerowitz EM: Early flower development in Arabidopsis. Plant Cell 2: 755-767 (1990).

    Google Scholar 

  13. Swanson MS, Dreyfuss G: Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol 8: 2237-2241 (1988).

    Google Scholar 

  14. Thomas TL: Gene expression during plant embryogenesis and germination: an overview. Plant Cell 5: 1401-1410 (1993).

    Google Scholar 

  15. Varagona MJ, Schmidt RJ, Raikhel NV: Nuclear localization signal(s) required for nuclear targeting of the maize regulatory protein, Opaque-2. Plant Cell 4: 1213-1227 (1992).

    Google Scholar 

  16. Ye L, Sugiura M: Domains required for nucleic acid binding activities in chloroplast ribonucleoproteins. Nucl Acids Res 20: 6275-6279 (1992).

    Google Scholar 

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Hecht, V., Stiefel, V., Delseny, M. et al. A new Arabidopsis nucleic-acid-binding protein gene is highly expressed in dividing cells during development. Plant Mol Biol 34, 119–124 (1997). https://doi.org/10.1023/A:1005834402536

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  • DOI: https://doi.org/10.1023/A:1005834402536

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