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Effect of changing the endogenous concentration of auxins and cytokinins and the production of ethylene in pea stem cuttings on adventitious root formation

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Abstract

Decapitation or treatment with naphthylphthalamic acid (NPA) ndash; an inhibitor of IAA transport ndash; or a synthetic cytokinin N-(2-chloro-4-pyridyl)-N-phenylurea (CPPU) caused a decrease in rooting of pea cuttings. However the mode of action of the above treatments was different. Decapitation resulted in a decrease of indole-3-acetic acid (IAA) and a simultaneous increase in cytokinin content at the base of the cuttings. NPA decreased IAA even more, but did not influence cytokinins. CPPU alone or in combination with amino-ethoxy-vinylglycine (AVG) ndash; an ethylene biosynthesis inhibitor ndash; increased IAA at the rooting zone, but CPPU was transported from apex to the base of the cuttings where it inhibited rooting. NAA, applied alone after decapitation, stimulated rooting, probably partly by being an auxin, and partly by inhibiting the accumulation of cytokinins at the base of the cuttings. Treatment with AVG neither influenced rooting nor auxin or cytokinin content. Therefore, ethylene production does not seem to be one of the main factors involved in the reduced rooting after the various treatments.

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Authors and Affiliations

  1. Laboratory of Biology of Horticulture, Aristotelian University of Thessaloniki, Thessaloniki, 540 06, Greece

    M.A. Koukourikou-Petridou

  2. Institut fuuml;r Obst-, Gemuuml;se- und Weinbau, University of Hohenheim, 70593, Stuttgart, Germany

    F. Bangerth

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  1. M.A. Koukourikou-Petridou
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  2. F. Bangerth
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Koukourikou-Petridou, M., Bangerth, F. Effect of changing the endogenous concentration of auxins and cytokinins and the production of ethylene in pea stem cuttings on adventitious root formation. Plant Growth Regulation 22, 101–108 (1997). https://doi.org/10.1023/A:1005833311839

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