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Characterization of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants expressing NSM gene sequences

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Abstract

Transgenic Nicotiana tabacum plants expressing RNA sequences of the tomato spotted wilt virus NSM gene, which encodes the putative viral movement protein, were found to be highly resistant to infection with the virus. Expression of untranslatable as well as anti-sense RNA of the NSM gene resulted in resistance levels as high as those in plants expressing translatable RNA sequences. For all three types of transgenic plants resistance levels of up to 100% were reached in the S2 progeny. These results indicate that the resistance mediated by the NSM gene is accomplished by expression of transcripts rather than protein in transgenic plants, similar to previously observed N gene-mediated resistance. Protoplast inoculations revealed that resistant plants expressing NSM are, in contrast to N transgenic resistant plants, not resistant at the cellular level. This suggests the RNA-mediated resistance mechanism against TSWV targets viral mRNAs rather than the viral genome.

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Authors and Affiliations

  1. Department of Virology, Wageningen Agricultural University, Binnenhaven 11, P.O. Box 8045, 6709 PD, Wageningen, Netherlands

    Marcel Prins, Marjolein Kikkert, Cahya Ismayadi, William de Graauw & Rob Goldbach

  2. Division of Technology, S&G Seeds, Westeinde 62, P.O. Box 26, 1600 AA, Enkhuizen, Netherlands

    Peter de Haan

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  1. Marcel Prins
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  2. Marjolein Kikkert
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  3. Cahya Ismayadi
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  4. William de Graauw
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  5. Peter de Haan
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  6. Rob Goldbach
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Prins, M., Kikkert, M., Ismayadi, C. et al. Characterization of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco plants expressing NSM gene sequences. Plant Mol Biol 33, 235–243 (1997). https://doi.org/10.1023/A:1005729808191

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