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Modulation at a Distance of Proton Conductance through the Saccharomyces cerevisiae Mitochondrial F1F0-ATP Synthase by Variants of the Oligomycin Sensitivity-Conferring Protein Containing Substitutions near the C-Terminus

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Abstract

We have sought to elucidate how the oligomycin sensitivity-conferring protein (OSCP) of the mitochondrial F1F0-ATP synthase (mtATPase) can influence proton channel function. Variants of OSCP, from the yeast Saccharomyces cerevisiae, having amino acid substitutions at a strictly conserved residue (Gly166) were expressed in place of normal OSCP. Cells expressing the OSCP variants were able to grow on nonfermentable substrates, albeit with some increase in generation time. Moreover, these strains exhibited increased sensitivity to oligomycin, suggestive of modification in functional interactions between the F1 and F0 sectors mediated by OSCP. Bioenergetic analysis of mitochondria from cells expressing OSCP variants indicated an increased respiratory rate under conditions of no net ATP synthesis. Using specific inhibitors of mtATPase, in conjunction with measurement of changes in mitochondrial transmembrane potential, it was revealed that this increased respiratory rate was a result of increased proton flux through the F0 sector. This proton conductance, which is not coupled to phosphorylation, is exquisitely sensitive to inhibition by oligomycin. Nevertheless, the oxidative phosphorylation capacity of these mitochondria from cells expressing OSCP variants was no different to that of the control. These results suggest that the incorporation of OSCP variants into functional ATP synthase complexes can display effects in the control of proton flux through the F0 sector, most likely mediated through altered protein—protein contacts within the enzyme complex. This conclusion is supported by data indicating impaired stability of solubilized mtATPase complexes that is not, however, reflected in the assembly of functional enzyme complexes in vivo. Given a location for OSCP atop the F1-α3β3 hexamer that is distant from the proton channel, then the modulation of proton flux by OSCP must occur “at a distance.” We consider how subtle conformational changes in OSCP may be transmitted to F0.

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Author notes

  1. Glen M. Boyle

    Present address: Queensland Institute of Medical Research, Brisbane, Queensland, Australia

  2. Xavier Roucou

    Present address: Serono Pharmaceutical Research Institute, Geneva, Switzerland

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Monash University, PO Box 13D, Victoria, 3800, Australia

    Glen M. Boyle, Xavier Roucou, Phillip Nagley, Rodney J. Devenish & Mark Prescott

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  1. Glen M. Boyle
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Boyle, G.M., Roucou, X., Nagley, P. et al. Modulation at a Distance of Proton Conductance through the Saccharomyces cerevisiae Mitochondrial F1F0-ATP Synthase by Variants of the Oligomycin Sensitivity-Conferring Protein Containing Substitutions near the C-Terminus. J Bioenerg Biomembr 32, 595–607 (2000). https://doi.org/10.1023/A:1005674628249

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