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Construction of multi-chimeric pyrroloquinoline quinone glucose dehydrogenase with improved enzymatic properties and application in glucose monitoring

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Abstract

A multi-chimeric enzyme was constructed by combining the protein regions responsible for the enzymatic properties of Escherichia coli and Acinetobacter calcoaceticus pyrroloquinoline quinone glucose dehydrogenase (PQQGDH). The constructed multi-chimeric PQQGDH showed increased co-factor binding stability, thermal stability, an alteration in substrate specificity and a 10-fold increase in the K m value for glucose compared with the wild-type E. coli PQQGDH. The cumulative effect of each introduced protein region on the improvement of enzymatic properties was observed. The application of the multi-chimeric PQQGDH in amperometric glucose sensor construction achieved an expanded dynamic range together with increased operational stability and narrower substrate specificity. The glucose sensor can measure glucose from 5 to 40 mM, suggesting its potential for the direct measurement of high blood-glucose levels in diabetic patients.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei, Tokyo , 184-8588, Japan

    Hiromi Yoshida, Tsuyoshi Iguchi & Koji Sode

Authors
  1. Hiromi Yoshida
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  2. Tsuyoshi Iguchi
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  3. Koji Sode
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Yoshida, H., Iguchi, T. & Sode, K. Construction of multi-chimeric pyrroloquinoline quinone glucose dehydrogenase with improved enzymatic properties and application in glucose monitoring. Biotechnology Letters 22, 1505–1510 (2000). https://doi.org/10.1023/A:1005658316948

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  • DOI: https://doi.org/10.1023/A:1005658316948

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