Skip to main content
SpringerLink
Log in

Carcinogen-Induced, Free Radical-Mediated Reduction in Microsomal Membrane Fluidity: Reversal by Indole-3-propionic Acid

  • Published:
Journal of Bioenergetics and Biomembranes Aims and scope Submit manuscript

Abstract

Chromium (Cr) is a well established carcinogen, with Cr(III) accounting for much of the intracellular oxidative damage that this transition metal induces. Indole-3-propionic acid (IPA), a melatonin-related molecule, is a reported antioxidant and free radical scavenger. Concentration (1, 10, 100, 500, or 1000 μM) and time (15, 30, 45, 60, or 90 min)-dependent effects of Cr(III) in the presence of H2O2 (0.5 mM), as well as the protective effect of IPA on Cr(III)-induced alterations in membrane fluidity (the inverse of membrane rigidity), as an index of membrane damage, were estimated by fluorescence spectroscopy. Cr(III), in a concentration- and a time-dependent manner, decreased membrane fluidity, with marked effects at a concentration of 500 μM and 60 min of incubation. IPA (5, 3, or 1 mM) prevented the Cr(III)-induced decrease in membrane fluidity. It is concluded that the carcinogen Cr(III), in the presence of H2O2, generates free radicals, which decrease membrane fluidity in rat microsomal membranes. Membrane alterations are pharmacologically prevented by the antioxidant IPA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  • Arancia, G. and Donelli, G. (1991). Pharmacol. Res. 24, 205–217.

    PubMed  Google Scholar 

  • Belagyi, J., Pas, M., Raspor, P., Pesti M., and Pali, T. (1999). Biochim. Biophys. Acta 1421, 175–182.

    PubMed  Google Scholar 

  • Blask, D. E., Sauer, L. A., Dauchy, R. T., Holowachuk, E. W., Ruhoff, M. S., and Kopff, H. S. (1999). Cancer Res. 59, 4693–4701.

    PubMed  Google Scholar 

  • Bradford, M. M. (1976). Anal. Biochem. 72, 248–254.

    Article  PubMed  Google Scholar 

  • Burcham, P. C. (1998). Mutagenesis 13, 287–305.

    PubMed  Google Scholar 

  • Candeias, L. P., Folkes, L. K., Rorssa, M., Parrick, J., and Wardman, P. (1995). Free Radical Res. 23, 403–418.

    Google Scholar 

  • Cheeseman, K. H. (1993). In DNA and Free Radicals (Halliwell, B. and Aruoma, O. I., eds), Ellis Horwood, London, pp. 107–144.

    Google Scholar 

  • Chen, J. J. and Yu, B. P. (1994). Free Radical Biol. Med. 17, 411–418.

    Google Scholar 

  • Chyan, Y.-J., Poeggeler, B., Omar, R. A., Chain, D. G., Frangione, B., Ghiso, J., and Pappolla, M. A. (1999). J. Biol. Chem. 274, 21937–21942.

    PubMed  Google Scholar 

  • Cohen, M., Kargacin, B., Klein, C. B., and Costa, M. (1993). Critical Rev. Toxicol. 23, 255–281.

    Google Scholar 

  • Denniston, M. L. and Uyeki, E. M. (1987). J. Toxicol. Environ. Health 21, 375–386.

    PubMed  Google Scholar 

  • Garcia, J. J., Reiter, R. J., Guerrero, J. M., Escames, G., Yu, B. P., Oh, C. S., and Munoz-Hoyos, A. (1997). FEBS Lett. 408, 297–300.

    PubMed  Google Scholar 

  • Garcia, J. J., Reiter, R. J., Ortiz, G. G., Oh, C. S., Tang, L., Yu, B. P., and Escames, G. (1998). J. Membr. Biol. 162, 59–65.

    PubMed  Google Scholar 

  • Garcia, J. J., Reiter, R. J., Pie, J., Ortiz, G. G., Cabrera, J., Sáinz, R. M., and Acuña-Castroviejo, D. (1999). J. Bioenerg. Biomembr. 31, 609–616.

    PubMed  Google Scholar 

  • Halliwell, B. (1992). J. Neurochem. 59, 1609–1623.

    PubMed  Google Scholar 

  • Hardeland, R., Zsizsik, B. K., Poeggeler, B., Fuhrberg, B., Holst, S., and Coto-Montes, A. (1999). Advan. Exp. Med. Biol. 467, 389–395.

    Google Scholar 

  • IARC Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. Chromium, Nickel and Welding (1990). Vol. 49 (IARC, Lyon, France), pp. 49–508.

  • Karbownik, M., Tan, D.-X., and Reiter, R. J. (2000a). Intern. J. Cancer 88, 7–11.

    Google Scholar 

  • Karbownik, M., Reiter, R. J., Garcia, J. J., Tan, D.-X., Qi, W., and Manchester, L. C. (2000b). Biochim. Biophys. Acta, 1523, 140–146.

    PubMed  Google Scholar 

  • Karbownik, M., Tan, D.-X., Manchester, L. C., and Reiter, R. J. (2000c). Cancer Lett., in press.

  • Lloyd, D. R., Carmichael, P. L., and Phillips, D. H. (1998). Chem. Res. Toxicol. 11, 420–427.

    PubMed  Google Scholar 

  • Morita, I., Kawamoto, M., and Yoshida, H. (1992). J. Chromatogr. 576, 334–339.

    PubMed  Google Scholar 

  • Pesti, M., Gazdag, Z., and Belagyi, J. (2000). FEMS Microbiol. Lett. 182, 375–380.

    PubMed  Google Scholar 

  • Petranka, J., Baldwin, W., Biermann, J., Yayadev, S., Barret, J. C., and Murphy, E. (1999). J. Pineal Res. 26, 129–136.

    PubMed  Google Scholar 

  • Poeggeler, B., Pappolla, M. A., Hardeland, R., Rassoulpour, A., Hodgkins, P. S., Guidetti, P., and Schwarcz, R. (1999). Brain Res. 815, 382–388.

    PubMed  Google Scholar 

  • Qi, W., Reiter, R. J., Tan, D.-X., Manchester, L. C., Karbownik, M., and Calvo, J. R. (2000a). Environ. Health Perspect. 108, 399–402.

    PubMed  Google Scholar 

  • Qi, W., Reiter, R. J., Tan, D.-X., Manchester, L. C., Siu, A. W., and Garcia, J. J. (2000b). J. Pineal Res. 29, 54–61.

    PubMed  Google Scholar 

  • Reiter, R. J. (1997). Advan. Pharmacol. 38, 103–117.

    Google Scholar 

  • Reiter, R. J. (1998). Progr. Neurobiol. 56, 359–384.

    PubMed  Google Scholar 

  • Reiter, R. J. (1999). Neuroendocrinol. Lett. 10: 145–150.

    Google Scholar 

  • Reiter, R. J., Tan, D.-X., Qi, W., Manchester, L. C., Karbownik, M., and Calvo, J. R. (2000a). Biol. Signals Recept. 9, 160–171.

    PubMed  Google Scholar 

  • Reiter, R. J., Tan, D. X., Osuna, C., and Gitto, E. (2000b). J. Biomed. Sci., 7, 444–458.

    PubMed  Google Scholar 

  • Romero, M. P., Osuna, C., Garcia-Pergañeda, Carillo-Vico, A., and Guerrero, J. M. (1999). J. Pineal Res. 26, 227–235.

    PubMed  Google Scholar 

  • Shi, X., Dalal, N. S., and Kasprzak, K. S. (1993). Arch. Biochem. Biophys. 302, 294–299.

    PubMed  Google Scholar 

  • Snow, E. T. (1992). Pharmacol. Therap. 53, 31–65.

    Google Scholar 

  • Tan, D. X., Manchester, L. C., Reiter, R. J., Qi, W., Karbownik, M., and Calvo, J. R. (2000a). Biol. Signals Recept. 9, 137–159.

    PubMed  Google Scholar 

  • Tan, D. X., Manchester, L. C., Reiter, R. J., Plummer, B. F., Linson, J., Weintraub, S. T., and Qi, W. (2000b). Free Radical Biol. Med., 29, 1177–1185.

    Google Scholar 

  • Tsou, T.-C, Chen, C.-L., Liu, T.-Y., and Yang, J.-L. (1996). Carcinogenesis 17, 103–108.

    PubMed  Google Scholar 

  • Young, S., Anderson, G. M., Gauthier, S., and Purdy, W. C. (1980). J. Neurochem. 34, 1087–1092.

    PubMed  Google Scholar 

  • Yu, B. P., Suescun, E. A., and Yang, S. Y. (1992). Mech. Aging Develop. 65, 17–33.

    Google Scholar 

  • Zhitkovich, A., Voitkun, V., and Costa, M. (1996). Biochemistry 35, 7275–7282.

    Article  PubMed  Google Scholar 

Download references

Authors
  1. Małgorzata Karbownik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Joaquin J. Garcia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andrzej Lewiński
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Russel J. Reiter
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Karbownik, M., Garcia, J.J., Lewiński, A. et al. Carcinogen-Induced, Free Radical-Mediated Reduction in Microsomal Membrane Fluidity: Reversal by Indole-3-propionic Acid. J Bioenerg Biomembr 33, 73–78 (2001). https://doi.org/10.1023/A:1005628808688

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005628808688

Search

Navigation