Abstract
A production system of UDP-N-acetylglucosamine (UDP-GlcNAc) was established by using recombinant Escherichia coli and Corynebacterium ammoniagenes in combination. E. coli overexpressed the UDP-GlcNAc biosynthetic genes, glmM, glmU, glk, ppa, ack, and pta, whereas C. ammoniagenes contributed to the formation of UTP from orotic acid. Glucose 1,6-diphosphate (Glc-1,6-P2), which was required for the activity of phosphoglucosamine mutase involved in UDP-GlcNAc biosynthesis, was supplied by phosphoglucomutase and phosphofructokinase. Starting with orotic acid (65 mM) and glucosamine (400 mM), UDP-GlcNAc accumulated at 11.4 mM (7.4 g l−1) after 8 h.
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Tabata, K., Koizumi, S., Endo, T. et al. Production of UDP-N-acetylglucosamine by coupling metabolically engineered bacteria. Biotechnology Letters 22, 479–483 (2000). https://doi.org/10.1023/A:1005627820455
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DOI: https://doi.org/10.1023/A:1005627820455