Abstract
Microbial production of bioactive glucosides using uridine diphosphate glucosyltransferase (UGT) is an efficient glucoside production method. Here, we describe a detailed method for the construction of a UDP-glucose biosynthetic enzyme gene coexpression plasmid, that is, pCDF-PGP and the microbial production of prunasin from racemic mandelonitrile using Escherichia coli possessing UGT85A47 obtained from Japanese apricot. Furthermore, this constructed vector can find application in the production of various other glucosides that utilize other UGTs and aglycons.
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Acknowledgments
This study was supported by the Exploratory Research for Advanced Technology Program (ERATO) Asano Active Enzyme Molecule Project from the Japan Science and Technology Agency (Grant number: JPMJER1102). This research was also supported in part by a grant-in-aid for Scientific Research (S) from The Japan Society for Promotion of Sciences (Grant number: 17H06169) to Y. Asano.
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Yamaguchi, T., Asano, Y. (2022). Construction of the UDP-Glucose Biosynthetic Enzyme Gene Coexpression Plasmid for Prunasin Production in Escherichia coli . In: Fett-Neto, A.G. (eds) Plant Secondary Metabolism Engineering. Methods in Molecular Biology, vol 2469. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2185-1_2
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DOI: https://doi.org/10.1007/978-1-0716-2185-1_2
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