Skip to main content
SpringerLink
Log in

Improved method for transport of living cell cultures

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

An easy and cost-effective method for transport of living cell cultures which avoids the use of dry ice and prevents bacterial contamination is described. Cells are suspended in buffered culture medium in sealed and insulated 2 ml cryovials and are able to grow and survive in substantial numbers during several days of storage and shipment at ambient temperature. Replating results in an identical repopulation in all cell lines. Not only tumor cells but also fibroblasts seem to tolerate well this improved method for shipment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barth RF (1998) Rat brain tumor models in experimental neurooncology: the 9L, C6, T9, F98, RG2 (D74), RT-2 and CNS-1 gliomas. J. Neurooncol. 36: 91-102.

    Google Scholar 

  • Danos O, Mulligan RC (1988) Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc. Natl. Acad. Sci. USA 85: 6460-6464.

    Google Scholar 

  • Simione FP, Brown EM(1991) ATCC Preservation Methods: Freezing and Freeze-drying, 2nd edition, Rockville, MD: ATCC.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurosurgery, Laboratory of Molecular Oncology, Martin-Luther-University Halle-Wittenberg, Halle, Germany

    Nikolai G. Rainov, Christel Trümpler & Ariel Quinones

  2. Gene Therapy Program, Translation Cancer Research Program, UCSD Cancer Center, Radiation Oncology, University of California at San Diego, San Diego, CA, 92103, USA

    Matthew A. Spear

  3. Department of Pediatrics, Heinrich-Heine-University, Düsseldorf, Germany

    Christof M. Kramm

Authors
  1. Nikolai G. Rainov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christel Trümpler
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ariel Quinones
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Matthew A. Spear
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Christof M. Kramm
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rainov, N.G., Trümpler, C., Quinones, A. et al. Improved method for transport of living cell cultures. Biotechnology Letters 22, 383–385 (2000). https://doi.org/10.1023/A:1005624718463

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005624718463

Search

Navigation