Abstract
Physiologically, healthy culture of cells is the basic need of present mammalian cell culture. During mammalian cell culture, the engaged laboratory personnel may face several troubles/challenges and problems. So, several cautionary measures are necessary to follow to overcome these trouble/challenges and problems. This chapter describes some routine trouble/challenges or problems being encountered by cell culture personnel, amidst the day-to-day laboratory works and the possible remedies to counter these hindrances. The major trouble, a mammalian cell culture personnel is likely to observe involves the changes in the pH of the cell culture medium, nonadherence or null attachment of adherent mammalian cells with the culture vessel surfaces, null or slow cellular growth, clumping of suspension cells in culture, programmed death (apoptosis) of the cultured cells, difficulty in recovering the cells from a cell bank, problems associated with the efficient cell transfection, and finally, contamination of mammalian cell culture with various microorganisms such as mycoplasma, bacteria, fungus, protozoa, and viruses. The success of mammalian cell culture depends upon the understanding of all the basic troubles associated with mammalian cell culture and concurrent effective troubleshooting.
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Mukherjee, S., Malik, P., Mukherjee, T.K. (2023). Troubleshooting of Mammalian Cell Culture. In: Mukherjee, T.K., Malik, P., Mukhopadhyay, S. (eds) Practical Approach to Mammalian Cell and Organ Culture. Springer, Singapore. https://doi.org/10.1007/978-981-19-1731-8_24-1
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DOI: https://doi.org/10.1007/978-981-19-1731-8_24-1
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Troubleshooting of Mammalian Cell Culture- Published:
- 07 January 2023
DOI: https://doi.org/10.1007/978-981-19-1731-8_24-2
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Troubleshooting of Mammalian Cell Culture- Published:
- 14 December 2022
DOI: https://doi.org/10.1007/978-981-19-1731-8_24-1