Abstract
Alkyl-β-glucosides were synthesized with β-glucosidase, from almond, in an amphiphilic phase reaction system using different water-miscible cosolvents. By means of stepwise addition of glucose, we obtained about 1.5 times higher concentration of hexylglucoside than that of normal reaction. Using the same way, octyl-, decyl- and dodecylglucoside were synthesized and their concentrations were 6.64, 5.3 and 4.48 g l−1, respectively.
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References
Beecher JE, Andrews AT, Vulfson EN (1990) Glycosidases inorganic solvents: II. Transgalactosylation catalysed by polyethyleneglycol-modified _-galactosidase. Enzyme Microbiol.Tech. 12: 955–959.
Chahid Z, Montet D, Pina M, Bonnot F, Graille J (1994) Biocatalyzedoctylglycoside synthesis from a disaccharide. Biotechnol.Lett. 16: 795–800.
Chahid Z, Montet D, Pina M, Graille J (1992) Effect of water activityon enzymatic synthesis of alkylglycosides. Biotechnol. Lett. 14: 281–284.
Dintinger T, Bouedec DD, Bouchonneau M, Tellier C (1994) Synthesisof _-mercaptoethyl-glycosides by enzymatic reverse hydrolysisand transglycosylation. Biotechnol. Lett. 16: 689–692.
Laroute V, Willemot RM (1992) Glucoside synthesis by glucoamylaseor _-glucosidase in organic solvents. Biotechnol. Lett. 14:169–174.
Nilsson KGI, Mayoralas AF (1991) _-D-Galactosidase-catalysedsynthesis of partially protected _-linked digalactopyranosides.Biotechnol. Lett. 13: 715–720.
Rosevear P, Vanaken T, Baxter J, Miller SF (1980) Alkyl glycosidedetergent: A simpler synthesis and their effects on kinetic andphysical properties of cytochrome c oxidase. J. Biochem. 19:4108–4115.
Selisko B, Ulbrich R, Schellenberger A (1989) Invertase-catalyzedreaction in alcoholic solutions. Biotechnol. Bioeng. 35: 1006–1010.
Stevenson DE, Stanley RA, Furneaux RH (1993) Optimization ofalkyl _-D-galactopyranoside synthesis from lactose using commerciallyavailable _-galactosidases. Biotechnol. Bioeng. 42:657–666.
Trincone A, Nicolaus B, Lama L, Morzillo P, RosaMD, GambacortaA (1991) Enzyme-catalyzed synthesis of alkyl _-D-glucosideswith crude homogenate of sulfolobus solfataricus. Biotechnol.Lett. 13: 235–240.
Tsitsimpikou C, Voutou D, Christakopoulos P, Kekos D, MacrisBJ, Kolisis FN (1994) Studies of the effect of organic solventson the stability of _-glucosidase from Fusarium oxysporum.Biotechnol. Lett. 16: 57–62.
Vulfson EN, Patel R, Law BA (1990) Alkyl-_-glucoside synthesisin a water-organic two-phase system. Biotechnol. Lett. 12: 397–402.
Vulfson EN, Patel R, Beecher PJ, Andrews AT, Law BA (1990)Glycosidases in organic solvents: I. Alkyl-_-glucoside synthesisin a water-organic two-phase system. Enzyme Microbiol. Tech. 12: 950–954.
Yi Q, Sarney DB, Khan JA, Vulfson EN (1998) A novel approachto biotransformations in aqueous-organic two-phase systems:Enzymatic synthesis of alkyl _-[D]-glucosides using microencapsulated_-glucosidase. Biotechnol. Bioeng. 60: 385–390.
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Park, DW., Kim, HS., Jung, JK. et al. Enzymatic synthesis of alkylglucosides by amphiphilic phase enzyme reaction. Biotechnology Letters 22, 951–956 (2000). https://doi.org/10.1023/A:1005620707624
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DOI: https://doi.org/10.1023/A:1005620707624