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Quantification of carotenoids in cells of Phaffia rhodozyma by autofluorescence

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Abstract

The distribution and intensity of autofluorescence caused by carotenoids in the yeast Phaffia rhodozyma was examined by laser confocal fluorescence microscopy. Carotenoid contents of individual yeast cells could be calculated from the value of autofluorescence/unit volume of yeast cell. The average level of fluorescence in highly fluorescing yeast cells was 15 mg carotenoid g−1 yeast which was accepted as the maximum yield of carotenoid in P. rhodozyma. The method developed in this study is useful for the research of cell biology associated with the biological functions of carotenoids in individual cells.

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Authors and Affiliations

  1. Department of Applied Biological Sciences, Sunmoon University, 100 Kalsan-Ri Tangjeong-Myon, Asan

    G.-H. An, H.-C. Kwon & K. Kim

  2. Rural Development Administration, #250, Sodun-dong, Kwonsun-gu, Suwon, Kyungki , 440-100, Korea

    O.-S. Suh

  3. Department of Food Microbiology and Toxicology, University of Wisconsin, 1925 Willow Drive, Madison

    E.A. Johnson

Authors
  1. G.-H. An
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  2. O.-S. Suh
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  3. H.-C. Kwon
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  4. K. Kim
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  5. E.A. Johnson
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An, GH., Suh, OS., Kwon, HC. et al. Quantification of carotenoids in cells of Phaffia rhodozyma by autofluorescence. Biotechnology Letters 22, 1031–1034 (2000). https://doi.org/10.1023/A:1005614010003

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  • DOI: https://doi.org/10.1023/A:1005614010003

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