Increased taxane accumulation in callus cultures of Taxus cuspidata and Taxus × media by some elicitors and precursors

Abstract

In Taxus cuspidata callus, vanadyl sulfate (10 mg l⁻¹) induced a high (146 μg g⁻¹ dry wt) production of 10-deacetylbaccatin III in comparison to 7 μg g⁻¹ dry wt of the control. The content of paclitaxel in this species increased from 16 μg g⁻¹ to 74 μg g⁻¹ dry wt when 20 mg phenylalanine l⁻¹ was used. In T. media, p-aminobenzoic acid induced the highest content of 10-deacetylbaccatin III (481 μg g⁻¹ dry wt) versus 181 μg g⁻¹ in the control. Paclitaxel increased from 89 to 139 μg  g⁻¹ dry wt after adding chitosan (20 mg l⁻¹) to the cultures.