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An esterification method for determination of lipase activity

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Abstract

The choice of a lipase for an esterification reaction can be determined from the esterification reaction between butyric acid (0.16 M) and butanol (0.33 M) at 50 °C and agitated with 10 mg lipase. The decrease in butyric acid is measured by titration and 1 unit of lipase activity is defined as 1 μmol butyric acid consumed per min per mg lipase.

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Authors and Affiliations

  1. Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore, 570 013, India

    K.R. Kiran, S. Hari Krishna, C.V. Suresh Babu, N.G. Karanth & S. Divakar

Authors
  1. K.R. Kiran
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  2. S. Hari Krishna
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  3. C.V. Suresh Babu
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  4. N.G. Karanth
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  5. S. Divakar
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Kiran, K., Hari Krishna, S., Suresh Babu, C. et al. An esterification method for determination of lipase activity. Biotechnology Letters 22, 1511–1514 (2000). https://doi.org/10.1023/A:1005604206258

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