Abstract
Dioscorea deltoidea cell suspension cultures were established in modified Murashige and Skoog medium. The diosgenin production increased from 0.10 g−1 to 3.98 g−1 dry cell weight when cells were cultivated in the light and in a growth medium limited in phosphate and sucrose. The addition of 1.3 g of autoclaved fungal mycelium of Alternaria tenuis per litre of cell culture growing in the dark induced the production of 0.04 mg diosgenin g−1 dry cell weight. In both cases, the production of diosgenin was preceded by a transient induction of isopentenyl diphosphate isomerase activity.
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Rojas, R., Alba, J., Magaña-Plaza, I. et al. Stimulated production of diosgenin in Dioscorea galeottiana cell suspension cultures by abiotic and biotic factors. Biotechnology Letters 21, 907–911 (1999). https://doi.org/10.1023/A:1005598623728
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DOI: https://doi.org/10.1023/A:1005598623728