Abstract
In the presence of a H2O2-generating system, the peroxidase of Pleurotus ostreatus that decolorise Remazol Brilliant Blue R catalysed the partial depolymerisation of lignosulfonate. The UV spectrum of the lignosulfonate changed with time with the absorbance at 280 nm increasing gradually. Gel permeation profile showed that the pattern of molecular weight distribution was changed to a lower molecular weight region. Oxidation products were identified as 2,6-dimethoxy-1,4-benzoquinone (I), benzoic acid (II), butyl phthalate (III), and bis (2-ethylhexyl) phthalate (IV) by GC/MS procedures.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alder E (1977) Lignin chemistry — past, present and future. Wood Sci. Technol. 11: 169–218.
Chen CL (1988) Characterization of lignin by oxidative degradation: use of gas chromatography-mass spectrometry technique. Meth. Enzymol. 161: 110–136.
Glenn JK, Akileswaran L, Gold MH (1986) Mn (II) oxidation is the principal function of the extracellular Mn-peroxidase from Phanerochaete chrysosporium. Arch. Biochem. Biophys. 251: 688–696.
Glenn JK, Morgan MA, Mayfield MB, Kuwahara M, Gold MH (1983) An extracellular H2O2-requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete Phanerochaete chrysosporium. Biochem. Biophys. Res. Commun. 114: 1077–1083.
Han YH, Shin KS, Youn HD, Hah YC, Kang SO (1996) Mode of action and active site of an extracellular peroxidase from Pleurotus ostreatus. Biochem. J. 314: 421–426.
Higuchi T (1990) Lignin biochemistry: biosynthesis and biodegradation. Wood Sci. Technol. 24: 23–63.
Huynh VB, Paszczynski A, Olson P, Crawford R (1986) Transformation of arylpropane lignin model compounds by a lignin peroxidase of the white-rot fungus Phanerochaete chrysosporium. Arch. Biochem. Biophys. 250: 186–196.
Kang SO, Shin KS, Han YH, Youn HD, Hah YC (1993) Purification and characterisation of an extracellular peroxidase from white-rot fungus Pleurotus ostreatus. Biochim. Biophys. Acta 1163: 158–164.
Kirk TK, Farrell RL (1987) Enzymatic combustion: the microbial degradation of lignin. Annu. Rev. Microbiol. 41: 465–505.
Shin KS (1995) Oxidation of syringic acid by extracellular peroxidase of white-rot fungus, Pleurotus ostreatus. Mycoscience 36: 31–35.
Shin KS, Oh IK, Kim CJ (1997) Production and purification of remazol brilliant blue R decolorizing peroxidase from the culture filtrate of Pleurotus ostreatus. Appl. Environ. Microbiol. 63: 1744–1748.
Tien M, Kirk TK (1983) Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science 221: 661–663.
Ulmer DC, Leisola MSA, Schmidt BH, Fiechter A (1983) Rapid degradation of isolated lignins by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 45: 1795–1801.
Valli K, Gold MH (1991) Degradation of 2,4-dichlorophenol by the lignin-degrading fungus Phanerochaete chrysosporium. J. Bacteriol. 173: 345–352.
Wariishi H, Valli K, Gold MH (1989) Oxidative cleavage of a phenolic diarylpropane lignin model dimer by manganese peroxidase from Phanerochaete chrysosporium. Biochemistry 28: 6017–6023.
Wariishi H, Valli K, Gold MH (1991) In vitro depolymerization of lignin by manganese peroxidase of Phanerochaete chrysosporium. Biochem. Biophys. Res.Commun. 176: 269–275.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shin, KS., Lee, YJ. Depolymerisation of lignosulfonate by peroxidase of the white-rot basidiomycete, Pleurotus ostreatus . Biotechnology Letters 21, 585–588 (1999). https://doi.org/10.1023/A:1005591027897
Issue Date:
DOI: https://doi.org/10.1023/A:1005591027897