Skip to main content
SpringerLink
Log in

Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Aspergillus niger

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

The lignin peroxidase (isoenzyme H8) of Phanerochaete chrysosporium was expressed in Aspergillus niger under the control of plant nopaline synthase (NOS) promoter and terminator. H8 mRNA was produced in this heterologous system. Western blot analysis showed that the recombinant protein was of size similar to the native H8. The extracellular lignin peroxidase activity in these primary constructs was positive, though weak (1.12 nKat mg−1).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chambers SP, Prior SE, Barstow DA, Minton NP (1988) The pMTL nic-cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene 68: 139–149.

    Google Scholar 

  • Cove DJ (1966) The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim. Biophys. Acta 113: 51–56.

    Google Scholar 

  • Doyle WA, Smith AT (1996) Expression of lignin peroxidase H8 in Escherichia coli: folding and activation of the recombinant enzyme with Ca2+ and haem. Biochem. J. 315: 15–19.

    Google Scholar 

  • Gold MH, Kuwahara M, Chiu AA, Glenn JK (1984) Purification and characterisation of an extra-cellular H2O2-requiring diarylproapne oxygenase from the white rot basidiomecete Phanerochaete chrysosporium. Arch. Biochem. Biophys. 234: 353–362.

    Google Scholar 

  • Hamdi M, Bouhamed H, Ellouz R (1991) Optimisation of the fermentation of olive mill waste waters by Aspergillus niger. Appl. Microbiol. Biotechnol. 36: 285–288.

    Google Scholar 

  • Haemmerli SD, Leisola MS, Sanglard D, Fiechter A (1986) Oxidation of benzo(a) pyrene by extracellular ligninases of Phanerochaete chrysosporium. J. Biol. Chem. 261: 6900–6903.

    Google Scholar 

  • Johnson TM, Li JK (1991) Heterologous expression and characterisation of an active lignin peroxidase from Phanerochaete chrysosporium using recombinant baculovirus. Arch. Biochem. Biophys. 291: 371–378.

    Google Scholar 

  • Kersten PJ, Kalyanaraman B, Hammel KE, Reinhammar B, Kirk TK (1990) Comparison of lignin peroxidase, horseradish peroxidase and laccase in the oxidation of methoxybenzenes. Biochem. J. 268: 475–480

    Google Scholar 

  • Kirk TK, Schultz E, Connors WJ, Lorenz LF, Zeikus JG (1978) Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium. Arch. Microbiol. 117: 277–285.

    Google Scholar 

  • Leisola MS, Kozulic B, Meussdoerffer F, Fiechter A (1987) Homology among multiple extra-cellular peroxidases from Phanerochaete chrysosporium. J. Biol. Chem. 262: 419–424.

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrook J (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Mohr G, Esser K (1990) Improved transformation frequency and heterologous promoter recognition in Aspergillus niger. Appl. Microbiol. Biotechnol. 34: 63–67.

    Google Scholar 

  • Moukha SM, Wosten HA, Mylius EJ, Asther M, Wessels JG (1993) Spatial and temporal accumulation of mRNAs encoding two common lignin peroxidases in Phanerochaete chrysosporium. J. Bacteriol. 175: 3672–3678.

    Google Scholar 

  • Saloheimo M, Barajas V, Niku-Paavola ML, Knowles JK (1989) A lignin peroxidase encoding cDNA from the white-rot fungus Phlebia radiata: characterisation and expression in Trichoderma reesei. Gene 85: 343–351.

    Google Scholar 

  • Sayadi S, Ellouz R (1995) Roles of lignin peroxidase and manganese peroxidase from Phanerochaete chrysosporium in the decolarization of olive mill wastewaters. Appl. Env. Microbiol. 61: 1098–1103.

    Google Scholar 

  • Stewart P, Whitwam RE, Kersten PJ, Cullen D, Tien M (1996) Efficient expression of Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. Appl. Env. Microbiol. 62: 860–864.

    Google Scholar 

  • Tien M, Tu CPD (1987) Cloning and sequencing of a cDNA for a ligninase from Phanerochaete chrysosporium. Nature 326: 520–523.

    Google Scholar 

  • Tien M, Kirk TK (1988) Lignin peroxidase of Phanerochaete chrysosporium. Meth. Enzymol. 161: 238–249.

    Google Scholar 

  • Waldron C, Murphy EB, Roberts JL, Gustafson GD, Armour SL, Malcom SK (1985) Resistance to hygromycin B: a new marker for plant transformation studies. Plant Mol. Biol. 5: 103–108.

    Google Scholar 

  • Wernars K, Goosen T, Swart K, Hondel CAMJJ van den, Broek HWJ van den (1987) Cotransformation of Aspergillus nidulans: a tool for replacing fungal genes. Mol. Gen. Genet. 209: 71–77.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centre de Biotechnologie de Sfax, BP‘K’3018, Sfax, Tunisia

    Mohamed Sami Aifa, Sami Sayadi & Ali Gargouri

Authors
  1. Mohamed Sami Aifa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sami Sayadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ali Gargouri
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aifa, M.S., Sayadi, S. & Gargouri, A. Heterologous expression of lignin peroxidase of Phanerochaete chrysosporium in Aspergillus niger . Biotechnology Letters 21, 849–853 (1999). https://doi.org/10.1023/A:1005541206270

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005541206270

Search

Navigation