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Increase of catalytic activity of α-chymotrypsin in organic solvent by co-lyophilization with cyclodextrins

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Abstract

α-Chymotrypsin was lyophilized in the presence of 2,3,6-tri-O-methyl β-cyclodextrin, and it displayed activity 40 fold higher than free α-chymotrypsin for transesterification in acetonitrile. α-Chymotrypsin which was co-lyophilized with hydroxypropylated β- or γ-cyclodextrins retained more than 98% of its initial activity after 6 h incubation in acetonitrile.

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Authors and Affiliations

  1. Institute of Materials Science, University of Tsukuba, Tennoudai, Tsukuba, Ibaraki, 305-8573, Japan

    Yu Ooe, Shinji Yamamoto, Masami Kobayashi & Hideo Kise

Authors
  1. Yu Ooe
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  2. Shinji Yamamoto
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  3. Masami Kobayashi
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  4. Hideo Kise
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Ooe, Y., Yamamoto, S., Kobayashi, M. et al. Increase of catalytic activity of α-chymotrypsin in organic solvent by co-lyophilization with cyclodextrins. Biotechnology Letters 21, 385–389 (1999). https://doi.org/10.1023/A:1005447407774

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  • DOI: https://doi.org/10.1023/A:1005447407774

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