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Characterization and biobleaching effect of hemicellulases produced by thermophilic fungi

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Abstract

The culture supernatant of Thermomyces lanuginosus strains MED 2D and MED 4B1 had high activities of xylanase with low inducible activities of β-xylosidase. The crude xylanase was optimally active at 70 °C and at pH 6.0 to 6.5. Subsequently Eucalyptus kraft pulp was treated with MED 2D supernatant at 10 IU per gram pulp resulting in a 10.5% reduction in Kappa number. XECEDED-Refined bleached pulp resulted in handsheets with increased brightness compared to the control (X = xylanase treatment; E = alkaline extraction; C = Cl2 treatment; D = ClO2 treatment).

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Authors and Affiliations

  1. Department of Microbiology, University of Stellenbosch, Stellenbosch, 7600, South Africa

    J. Haarhoff, C.J. Moes, C. Cerff, W.J. van Wyk & G. Gerischer

  2. Mondi Forests, Hilton , 3245, South Africa

    B.J.H. Janse

Authors
  1. J. Haarhoff
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  2. C.J. Moes
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  3. C. Cerff
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  4. W.J. van Wyk
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  5. G. Gerischer
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  6. B.J.H. Janse
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Haarhoff, J., Moes, C., Cerff, C. et al. Characterization and biobleaching effect of hemicellulases produced by thermophilic fungi. Biotechnology Letters 21, 415–420 (1999). https://doi.org/10.1023/A:1005406010391

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