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Biotransformation of bezo[a]pyrene in bench scale reactor using laccase of Pycnoporus cinnabarinus

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Abstract

The biotransformation of benzo[a]pyrene by purified extracellular laccase of Pycnoporus cinnabarinus was investigated in bench scale reactors. The reaction required the presence of exogenous mediator ABTS. Most of 95% of the substrate was converted within 24 hours. The enzyme preparation oxidised the substrate mainly to benzo[a]pyrene 1,6- 3,6- and 6,12-quinones in a 2/1/1 ratio after 24 h incubation.

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Authors and Affiliations

  1. KUPKA batiment C, KREBS, 14 Rue Hoche - dfense, Paris, France

    Rachel Rama

  2. Unit de Phytopharmacie et Médiateurs Chimiques, Institut National de la Recherche Agronomique (INRA), Route de Saint-Cyr, Versailles, France

    Christian Mougin, François-Didier Boyer, Albert Kollmann, Christian Malosse & Jean-Claude Sigoillot

Authors
  1. Rachel Rama
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  2. Christian Mougin
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  3. François-Didier Boyer
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  4. Albert Kollmann
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  5. Christian Malosse
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  6. Jean-Claude Sigoillot
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Rama, R., Mougin, C., Boyer, FD. et al. Biotransformation of bezo[a]pyrene in bench scale reactor using laccase of Pycnoporus cinnabarinus. Biotechnology Letters 20, 1101–1104 (1998). https://doi.org/10.1023/A:1005387016390

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  • DOI: https://doi.org/10.1023/A:1005387016390

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