Skip to main content
SpringerLink
Log in

Enhancement of cell growth by intracellularly expressed herpes simplex virus thymidine kinase

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A recombinant cell line (NIH3T3:pLtkSN) was made by infecting parental cells (NIH3T3) with a recombinant retrovirus (pLtkSN) encoding herpes simplex virus thymidine kinase (HSVtk) gene. The cells expressing HSVtk (NIH3T3:pLtkSN) grew 2.3 times more than the parental cells (NIH3T3) in Dulbecco's Modified Eagles Media containing 10% (v/v) horse serum. The NIH3T3:pLtkSN cells also showed a significant enhancement in the maximal cell concentration and the specific growth rate even at 2.5% serum concentration. The specific O2 uptake rate of NIH3T3 was 2.1 times greater than that of NIH3T3:pLtkSN. Under both O2-limited and O2-unlimited conditions, it appears that HSVtk plays an important role in enhancing the growth characteristics of animal cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chen, C and Okayama, H (1987). Mol Cell Biol 7:2745-2752.

    Google Scholar 

  • Gudas, JM, Knight, GB and Pardee, AB (1990). Mol Cell Biol 10:5591-5595.

    Google Scholar 

  • Karlseder, J, Rotheneder, H and Wintersberger, E (1996). Mol Cell Biol 16:1659-1667.

    Google Scholar 

  • Knöfler, M, Waltner, C, Wintersber, E and M(81)llner, EW (1993). J Biol Chem 268:11409-11416.

    Google Scholar 

  • Morgan, JR, Lee, J, Tompkins, RG and Yarmush, ML (1994). Biotechnol Prog 10: 441-446.

    Google Scholar 

  • Park, SH and Yu, YS (1997). Keimyung Univ., Bull. I. I. S. 19-2(I):17-26.

    Google Scholar 

  • van der Pol., LA, Bonarius, D, van de Wouw, G. and Tramper, J (1993). Biotechnol Prog 9:504-509.

    Google Scholar 

  • Wawra, E, Pöckl, E, M(81)llner, E and Wintersber E (1981). J Virol 38:973-981.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Chemical Engineering and Materials Engineering, Keimyung University, Taegu, Korea

    Sun Ho Park & Yeon Su Yu

  2. Department of Biochemistry, School of Medicine, Catholic University of Taegu-Hyosung, Taegu, Korea

    Jongwon Lee

Authors
  1. Sun Ho Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yeon Su Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jongwon Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, S.H., Yu, Y.S. & Lee, J. Enhancement of cell growth by intracellularly expressed herpes simplex virus thymidine kinase. Biotechnology Letters 20, 105–108 (1998). https://doi.org/10.1023/A:1005355819614

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1005355819614

Keywords

Search

Navigation