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Secretory expression of human alpha1 -antitrypsin in Saccharomyces cerevisiae using galactose as a gratuitous inducer

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Abstract

Human alpha1 -antitrypsin (alpha1-AT) was expressed and secreted in Saccharomyces cerevisiae carrying the gal1 and reg1-501 mutations under the control of GAL10 promoter. Increasing galactose from 0 to 0.5 g/l increased the extracellular concentration of alpha1-AT, though the secretion efficiency was decreased. The final titre of alpha1-AT secreted and the secretion efficiency were 17.9 mu g/ml and 24.8% respectively, with 0.5 g galactose/l. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Korea Research Institute of Bioscience and Biotechnology, P. O. Box 115, Yusong Taejon, Korea

    Bong Hyun Chung, Su Jeong Kim, Hyun Ah Kang & Meyong-Hee Yu

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  1. Bong Hyun Chung
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  2. Su Jeong Kim
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  3. Hyun Ah Kang
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  4. Meyong-Hee Yu
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Chung, B.H., Kim, S.J., Kang, H.A. et al. Secretory expression of human alpha1 -antitrypsin in Saccharomyces cerevisiae using galactose as a gratuitous inducer. Biotechnology Letters 20, 307–311 (1998). https://doi.org/10.1023/A:1005350507744

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