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Exploring the Feasibility of the Sec Route to Secrete Proteins Using the Tat Route in Streptomyces lividans

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Abstract

Streptomyces lividans uses mainly two pathways to target secretory proteins to the cytoplasmic membrane. The major pathway (Sec pathway) transports pre-proteins using the signal recognition particle, and the minor Tat pathway is responsible for the secretion using a folded conformation of a relatively low number of proteins. The signal peptides of the Sec-dependent alpha-amylase and the Tat-dependent agarase were interchanged and fused in-frame to the corresponding mature part of the other enzyme. Alpha-amylase was unable to use the Tat route when fused to the agarase signal peptide, while agarase used the Sec route when it was targeted by the alpha-amylase signal peptide. In addition to the signal peptide some yet unidentified parts of the secreted proteins may play a role in selecting the secretory route. Structure predictions for the Tat- and Sec-dependent proteins suggest that less structured proteins are more likely to be candidates for the Tat route.

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Acknowledgments

The Spanish Ministry of the Environment and Rural and Marine affairs has commissioned and supported this research (Grant No. EGO22008). Grant PIE201220E003 from the CSIC also supported this research. We wish to thank C. Palomino for her contribution to obtaining S. lividans ∆tatC.

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Authors and Affiliations

  1. Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología (CNB-CSIC), c/Darwin 3, 28049, Madrid, Spain

    Sonia Gullón, Rebeca L. Vicente, José R. Valverde, Silvia Marín & Rafael P. Mellado

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  1. Sonia Gullón
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  2. Rebeca L. Vicente
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  3. José R. Valverde
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  4. Silvia Marín
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  5. Rafael P. Mellado
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Correspondence to Rafael P. Mellado.

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Gullón, S., Vicente, R.L., Valverde, J.R. et al. Exploring the Feasibility of the Sec Route to Secrete Proteins Using the Tat Route in Streptomyces lividans . Mol Biotechnol 57, 931–938 (2015). https://doi.org/10.1007/s12033-015-9883-0

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  • DOI: https://doi.org/10.1007/s12033-015-9883-0

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