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Hepatocytes cultured on lactose-substituted polystyrene become resistant to cytokine-induced cellular injury

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Abstract

Responses of hepatocytes on various extracellular matrices to injury by cytokine and endotoxin were studied. Hepatocytes cultured on poly-N-p-vinylbenzyl-D-lactonamide (PVLA) surfaces became resistant to IFN γ-induced cell injury and also resisted the stimulation from endotoxin and cytokines, and thus showed low nitric oxide synthesis ability. Damage to transplanted cell in engineered tissue/organ can thus be prevented by culturing cells on appropriate synthetic extracellular matrices.

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Authors and Affiliations

  1. National Key Laboratory of Biomembrane and Membrane Engineering, and Department of Biological Sciences and Biotechnology, School of Life Science and Engineering, Tsinghua University, Beijing, China

    Jun F. Liang

  2. Department of Biomolecular Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Yokohama, Japan

    Toshihiro Akaike

Authors
  1. Jun F. Liang
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  2. Toshihiro Akaike
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Liang, J.F., Akaike, T. Hepatocytes cultured on lactose-substituted polystyrene become resistant to cytokine-induced cellular injury. Biotechnology Letters 20, 173–176 (1998). https://doi.org/10.1023/A:1005336710044

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  • DOI: https://doi.org/10.1023/A:1005336710044

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