Abstract
Fifteen bacterial strains capable of utilizing naphthalene, phenanthrene, and biphenyl as the sole sources of carbon and energy were isolated from soils and bottom sediments contaminated with waste products generated by chemical- and salt-producing plants. Based on cultural, morphological, and chemotaxonomic characteristics, ten of these strains were identified as belonging to the genera Rhodococcus, Arthrobacter, Bacillus, and Pseudomonas. All ten strains were found to be halotolerant bacteria capable of growing in nutrient-rich media at NaCl concentrations of 1–1.5 M. With naphthalene as the sole source of carbon and energy, the strains could grow in a mineral medium with 1 M NaCl. Apart from being able to grow on naphthalene, six of the ten strains were able to grow on phenanthrene; three strains, on biphenyl; three strains, on octane; and one strain, on phenol. All of the strains were plasmid-bearing. The plasmids of the Pseudomonas sp. strains SN11, SN101, and G51 are conjugative, contain genes responsible for the degradation of naphthalene and salicylate, and are characterized by the same restriction fragment maps. The transconjugants that gained the plasmid from strain SN11 acquired the ability to grow at elevated NaCl concentrations. Microbial associations isolated from the same samples were able to grow at a NaCl concentration of 2.5 M.
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References
Yen, K.M. and Serdar, C.M., Genetic of Naphthalene Catabolism in Pseudomonads, CRC Crit. Rev. Microbiol., 1988, vol. 15, pp. 247–268.
Cerniglia, C.E., Biodegradation of Polycyclic Aromatic Hydrocarbons, Biodegradation, 1992, vol. 3, pp. 351–368.
Koronelli, T.V., Principles and Methods for Intensifying the Biodegradation of Hydrocarbons in the Environment. Prikl. Biokhim. Mikrobiol., 1996, vol. 32, no. 6, pp. 579–585.
Ventosa, A., Joaquhn, J.N., and Oren, A., Biology of Halophilic Aerobic Bacteria, Microbiol. Mol. Biol. Rev., 1998, vol. 2, p. 504.
Oren, A., Gurevich, P., Azachi, M., and Henis, Y., Microbial Degradation of Pollutants at High Salt Concentrations, Biodegradation, 1992, vol. 3, pp. 387–398.
Rozanova, E.P. and Nazina, T.N., Hydrocarbon-Oxidizing Bacteria and Their Activity in Oil Deposits, Mikrobiologiya, 1982, vol. 51, pp. 324–348.
Manual of Methods for General Bacteriology, Gerhardt, P. et al., Eds., Washington: Am. Soc. Microbiol., 1981. Translated under the title Metody obshchei bakteriologii, Moscow: Mir, 1983.
Chemical Methods in Bacterial Systematics, Goodfellow, M. and Minnikin, D.E., Eds., London: Academic, 1985.
Palleroni, N.J., Genus 1: Pseudomonas Migula, Bergey's Manual of Systematic Bacteriology, Krieg, N.R. and Holf, J.G., Eds., Baltimore: Williams and Wilkins, 1984, vol. 1, pp. 141–199.
Bergey's Manual of Systematic Bacteriology, Sneath, P.A. et al., Eds., Baltimore: Williams and Wilkins, 1984, vol. 2.
Birnboim, H.C. and Doly, J.A., A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA, Nucleic Acids Res., 1979, vol. 7, p. 1513.
Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab., 1982. Translated under the title Molekulyarnoe klonirovanie, Moscow: Mir, 1984.
Dunn, H.W. and Gunsalus, I.C., Transmissible Plasmids Coding Early Enzymes of Naphthalene Oxidation in Pseudomonas putida, J. Bacteriol., 1973, vol. 114, p. 974.
Rheinwald, J., Chakrabarty, A.M., and Gunsalus, I.C., A Transmissible Plasmids Controlling Camphor Oxidation in Pseudomonas putida, Proc. Natl. Acad. Sci. USA, 1973, vol. 70, pp. 885–889.
Osorio, K.R., Barja, J.L., Hutson, R.A., and Collins, M.D., Arthrobacter rhombi sp. nov., Isolated from Greenland Halibut (Reinhardtius hippoglossoides), Int. J. Syst. Bacteriol., 1999, vol. 49, pp. 1217–1220.
Schmitz, A., Gartemann, K.-H., Fiedler, J., Grund, E., Denecke, B., and Eichenlaub, R., Degradation of Polycyclic and Halogenated Aromatic Compounds by Rhodococcus and Arthrobacter Species, Proceeding of the Ninth Symposium on the Actinomycetes, 1995, pp. 150–154.
Microbial Life in Extreme Environments, Kushner, D.J., Ed., London: Academic, 1978. Translated under the title Zhizn' mikrobov v ekstremal'nykh usloviyakh, Moscow: Mir, 1981, p. 365.
Mueller, J.G., Devereux, R., Santavy, D.L., Lantz, S.E., Willis, S.G., and Pritchard, P.H., Phylogenetic and Physiological Comparison of PAH-degrading Bacteria from Geographically Diverse Soils, Antonie van Leeuwenhoek, 1997, vol. 71, pp. 329–343.
Kosheleva, I.A., Balashova, N.V., Izmalkova, T.Yu., Filonov, A.E., Sokolov, S.L., Slepen'kin, A.V., and Boronin, A.M., Degradation of Phenanthrene by Mutant Naphthalene-Degrading Pseudomonas putida Strains, Mikrobiologiya, 2000, vol. 69, no. 6, pp. 783–789.
Kiyohara, H. and Nagao, K., The Catabolism of Phenanthrene and Naphthalene by Bacteria, J. Gen. Microbiol., 1978, vol. 105, pp. 69–75.
Vreeland, R.H., Mechanisms of Halotolerance in Microorganisms, CRC Crit. Rev. Microbiol., 1987, vol. 14, pp. 311–356.
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Plotnikova, E.G., Altyntseva, O.V., Kosheleva, I.A. et al. Bacterial Degraders of Polycyclic Aromatic Hydrocarbons Isolated from Salt-Contaminated Soils and Bottom Sediments in Salt Mining Areas. Microbiology 70, 51–58 (2001). https://doi.org/10.1023/A:1004892804670
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DOI: https://doi.org/10.1023/A:1004892804670