Abstract
A culture of propionic acid bacteria grown in a glucose-containing minimal medium, as well as the culture liquid and logarithmic-phase cells obtained from this culture, were found to inhibit the base pair substitution mutations induced by 4-nitroquinoline N-oxide, N-methyl-N"-nitro-N-nitrosoguanidine, and sodium azide and the frameshift mutations induced by 9-aminoacridine. The antimutagenic activity of the culture liquid (CL) was presumably due to the presence of an extracellular thermolabile protein with a molecular mass of no more than 12 kDa, as evidenced by the facts that this activity considerably decreased after the treatment of the CL with pronase, its heating at 92°C, and its dialysis in a cellulose sack, which retains substances with molecular masses greater than 12 kDa. The residual antimutagenic activity of the dialyzed culture liquid was probably related to the interaction of the mutagen with thiols, rather than to the presence of organic acids (acetic or propionic). Thiols may also contribute to the antimutagenic activity of the Propionibacterium shermanii cells.
Similar content being viewed by others
References
Rao, D.R., Pulusani, S.R., and Chawan, C.B., Diet, Nutrition and Cancer: A Critical Evaluation. Vol. 11. Micronutrients, Nonnutritive Dietary Factors, and Cancer, Reddy, B.S. and Cohen, L.A., Eds., Boca Raton: CRC, 1986, pp. 63–75.
Shenderov, B.A. and Manvelova, M.A., Microbiological Implications of Functional Nutrition, Med. aspekty mikrobnoi ekologii (Medical Implications of Microbial Ecology), Shenderov, B.A., Ed., Moscow, 1994, no. 7/8, pp. 10–19.
Vorobjeva, L.I., Cherdinceva, T.A., Abilev, S.K., and Vorobjeva, N.V., Antimutagenicity of Propionic Acid Bacteria, Mutation Res., 1991, vol. 251, pp. 233–239.
Perez-Chaia, A., Zirate, G., and Oliver, G., The Probiotic Properties of Propionibacteria, Lait, 1999, vol. 79, pp. 175–185.
Sidorchuk, I.I. and Bondarenko, V.M., Selection of a Biologically Active Mutant of Propionibacterium shermanii and the Possibility of Its Use in Complex Therapy of Enteral Dysbacteriosis, J. Hyg. Epidemiol. Microbiol. Immunol., 1984, vol. 28, pp. 331–338.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J., Protein Measurement with the Folin Phenol Reagent, J. Biol. Chem., 1951, vol. 193, no. 1, pp. 265–275.
Maron, D.H. and Ames, B.N., Revised Methods for the Salmonella Mutagenicity Test, Handbook of Mutagenicity Test Procedures, Kilbey, B.J. et al., Eds., Amsterdam: Elsevier, 1984, 2nd ed., pp. 95–130.
Lankaputhra, W.E.V. and Shah, N.P., Antimutagenic Properties of Organic Acids from Probiotic Bacteria, Mutat. Res., 1998, vol. 397, pp. 169–182.
Matar, C., Nadathur, S.S., Bakalinsky, A.T., and Goulet, J., Antimutagenic Effects of Milk Fermented by Lactobacillus helveticus L819 and Protease-Deficient Derivative, J. Dairy Sci., 1997, vol. 80, pp. 1965–1970.
Osava, T.S., Namiki, M.S., Udake, S., and Kada, T., Chemical Studies of Antimutagens of Microbial Origin, Antimutagenesis and Anticarcinogenesis: Mechanisms, Shankel, D.M. et al., Eds., New York: Plenum, 1986.
Ketterer, B., Protective Role of Glutathione and Glutathione Transferase in Mutagenesis, Mutat. Res., 1988, vol. 202, pp. 343–361.
Vorob'eva, L.I., Stoyanova, L.G., and Alekseeva, M.A., Practical Use of Propionic Acid Bacteria, Mikrobnye metabolity (Microbial Metabolites), Zvyagintsev, D.G., Ed., Moscow: Mosk. Gos. Univ., 1979, pp. 88–100.
Vorobjeva, L.I., Khodjaev, E.Yu., and Cherdinceva, T.A., The Study of Induced Antimutagenesis of Propionic Acid Bacteria, J. Microbiol. Methods, 1996, vol. 24, pp. 249–258.
De Flora, S. and Ramel, C., Mechanisms of Inhibitors of Mutagenesis and Carcinogenesis: Classification and Overview, Mutat. Res., 1988, vol. 202, pp. 285–306.
Kuroda, Y. and Inoue, T., Antimutagenesis by Factors Affecting DNA Repair in Bacteria, Mutat. Res., 1988, vol. 202, pp. 387–391.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vorob'eva, L.I., Khodzhaev, E.Y. & Ponomareva, G.M. An Extracellular Protein of Propionic Acid Bacteria Inhibits Induced Mutations in Salmonella typhimurium Strains. Microbiology 70, 31–35 (2001). https://doi.org/10.1023/A:1004884502853
Issue Date:
DOI: https://doi.org/10.1023/A:1004884502853